TY - JOUR
T1 - Engineering a synthetic pathway in cyanobacteria for isopropanol production directly from carbon dioxide and light
AU - Kusakabe, Tamami
AU - Tatsuke, Tsuneyuki
AU - Tsuruno, Keigo
AU - Hirokawa, Yasutaka
AU - Atsumi, Shota
AU - Liao, James C.
AU - Hanai, Taizo
N1 - Funding Information:
This research was supported in part by JST , CREST . We would like to express gratitude to T. Kondo and Y. Kitayama (Nagoya University) for S. elongatus PCC7942, E.I. Lan (University of California, Los Angeles) and M. Aichi (Chubu University) for helpful advice, and C.A. Rabinovitch-Deere and J.T. McEwen (University of California, Davis) for language help.
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 2013/11
Y1 - 2013/11
N2 - Production of alternate fuels or chemicals directly from solar energy and carbon dioxide using engineered cyanobacteria is an attractive method to reduce petroleum dependency and minimize carbon emissions. Here, we constructed a synthetic pathway composed of acetyl-CoA acetyl transferase (encoded by thl), acetoacetyl-CoA transferase (encoded by atoAD), acetoacetate decarboxylase (encoded by adc) and secondary alcohol dehydrogenase (encoded by adh) in Synechococcus elongatus strain PCC 7942 to produce isopropanol. The enzyme-coding genes, heterogeneously originating from Clostridium acetobutylicum ATCC 824 (thl and adc), Escherichia coli K-12 MG1655 (atoAD) and Clostridium beijerinckii (adh), were integrated into the S. elongatus genome. Under the optimized production conditions, the engineered cyanobacteria produced 26.5. mg/L of isopropanol after 9 days.
AB - Production of alternate fuels or chemicals directly from solar energy and carbon dioxide using engineered cyanobacteria is an attractive method to reduce petroleum dependency and minimize carbon emissions. Here, we constructed a synthetic pathway composed of acetyl-CoA acetyl transferase (encoded by thl), acetoacetyl-CoA transferase (encoded by atoAD), acetoacetate decarboxylase (encoded by adc) and secondary alcohol dehydrogenase (encoded by adh) in Synechococcus elongatus strain PCC 7942 to produce isopropanol. The enzyme-coding genes, heterogeneously originating from Clostridium acetobutylicum ATCC 824 (thl and adc), Escherichia coli K-12 MG1655 (atoAD) and Clostridium beijerinckii (adh), were integrated into the S. elongatus genome. Under the optimized production conditions, the engineered cyanobacteria produced 26.5. mg/L of isopropanol after 9 days.
UR - http://www.scopus.com/inward/record.url?scp=84885166683&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84885166683&partnerID=8YFLogxK
U2 - 10.1016/j.ymben.2013.09.007
DO - 10.1016/j.ymben.2013.09.007
M3 - Article
C2 - 24076145
AN - SCOPUS:84885166683
SN - 1096-7176
VL - 20
SP - 101
EP - 108
JO - Metabolic Engineering
JF - Metabolic Engineering
ER -