TY - JOUR
T1 - Energy metabolism during cold ischemia and reperfusion in rat small intestinal transplantation
T2 - Comparison of jejunal and ileal grafts
AU - Nakao, Makoto
AU - Taguchi, Tomoaki
AU - Yanai, Keiko
AU - Yamada, Takaharu
AU - Suita, Sachiyo
N1 - Funding Information:
From the Department of Pediatric Surgery, Faculty of Medicine, Kyushu University, Fukuoka, Japan. This work was supported by a grant for pediatric research (W-02) from the Ministry of Health and Welfare, Japan. Address reprint requests to Sachiyo Suita, MD, Department of Pediatric Surgery, Faculty of Medicine, Kyushu University, 3-1-l. Maidashi, Higashi-ku, Fukuoka 812-82, Japan. Copyright o 1997 by WE. Saunders Company 0022-3468/97/3212-0003$03.00/O
PY - 1997/12
Y1 - 1997/12
N2 - Background/Purpose: In segmental small intestinal transplantation, the question as to whether the jejunum or ileum is the better graft remains unclear. The authors investigated this question regarding nutrition, blood chemistry, and adaptation. Methods: The authors compared the jejunum and ileum as the proper portion of segmental graft in rat small intestinal transplantation, regarding energy metabolism both during cold preservation and after reperfusion using high-performance liquid chromatography, as well as histological examination. Results: In a cold preservation study, the concentrations of adenosine triphosphate (ATP) in the jejunum versus ileum at 0, 6, 12, 24, and 48 hours of cold preservation were 1.98 ± 0.93 versus 1.83 ± 0.84, 0.79 ± 0.51 versus 0.55 ± 0.41, 0.60 ± 0.41 versus 0.58 ± 0.45, 0.38 ± 0.28 versus 0.47 ± 0.39, and 0.44 ± 0.26 versus 0.55 ± 0.29 (μmol/g weight), respectively. There were no significant differences between the jejunum and ileum at any times of preservation. Adenosine monophosphate (AMP), adenosine diphosphate (ADP), total adenine nucleotides (TAN), and energy charge (EC) also showed no significant differences between the jejunum and ileum at any times of preservation. In the reperfusion study, the concentrations of ATP in jejunum versus ileum 30 minutes after reperfusion after 6, 12, and 24 hours of cold preservation were 0.68 ± 0.19 versus 0.50 ± 0.17, 0.71 ± 0.51 versus 0.42 ± 0.14, and 0.93 ± 0.29 versus 0.75 ± 0.47 (μmol/g dry weight), respectively. Each value was slightly higher in the jejunum than that in the ileum; however, there were no statistically significant differences. TAN showed the same changes as ATP. No significant differences were found between the jejunum and ileum in either AMP, ADP, or EC. The histological findings both just before and 30 minutes after reperfusion were also compared. However, no evident differences were found between the jejunum and ileum. Conclusion: These results thus suggest that no significant differences exist between the jejunum and ileum regarding energy metabolism either during cold preservation or after reperfusion in small intestinal transplantation.
AB - Background/Purpose: In segmental small intestinal transplantation, the question as to whether the jejunum or ileum is the better graft remains unclear. The authors investigated this question regarding nutrition, blood chemistry, and adaptation. Methods: The authors compared the jejunum and ileum as the proper portion of segmental graft in rat small intestinal transplantation, regarding energy metabolism both during cold preservation and after reperfusion using high-performance liquid chromatography, as well as histological examination. Results: In a cold preservation study, the concentrations of adenosine triphosphate (ATP) in the jejunum versus ileum at 0, 6, 12, 24, and 48 hours of cold preservation were 1.98 ± 0.93 versus 1.83 ± 0.84, 0.79 ± 0.51 versus 0.55 ± 0.41, 0.60 ± 0.41 versus 0.58 ± 0.45, 0.38 ± 0.28 versus 0.47 ± 0.39, and 0.44 ± 0.26 versus 0.55 ± 0.29 (μmol/g weight), respectively. There were no significant differences between the jejunum and ileum at any times of preservation. Adenosine monophosphate (AMP), adenosine diphosphate (ADP), total adenine nucleotides (TAN), and energy charge (EC) also showed no significant differences between the jejunum and ileum at any times of preservation. In the reperfusion study, the concentrations of ATP in jejunum versus ileum 30 minutes after reperfusion after 6, 12, and 24 hours of cold preservation were 0.68 ± 0.19 versus 0.50 ± 0.17, 0.71 ± 0.51 versus 0.42 ± 0.14, and 0.93 ± 0.29 versus 0.75 ± 0.47 (μmol/g dry weight), respectively. Each value was slightly higher in the jejunum than that in the ileum; however, there were no statistically significant differences. TAN showed the same changes as ATP. No significant differences were found between the jejunum and ileum in either AMP, ADP, or EC. The histological findings both just before and 30 minutes after reperfusion were also compared. However, no evident differences were found between the jejunum and ileum. Conclusion: These results thus suggest that no significant differences exist between the jejunum and ileum regarding energy metabolism either during cold preservation or after reperfusion in small intestinal transplantation.
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U2 - 10.1016/S0022-3468(97)90504-2
DO - 10.1016/S0022-3468(97)90504-2
M3 - Article
C2 - 9433997
AN - SCOPUS:0031455387
SN - 0022-3468
VL - 32
SP - 1675
EP - 1678
JO - Journal of Pediatric Surgery
JF - Journal of Pediatric Surgery
IS - 12
ER -