Endothelin type B receptor-induced sustained Ca2+ influx involves Gq/11/phospholipase C-independent, p38 mitogen-activated protein kinase-dependent activation of Na+/H+ exchanger

Tsunaki Higa; Takahiro Horinouchi; Hiroyuki Aoyagi; Hiroshi Asano; Tadashi Nishiya; Arata Nishimoto; Ikunobu Muramatsu; Soichi Miwa

doi:10.1254/jphs.10102SC

Endothelin type B receptor-induced sustained Ca²⁺ influx involves G_q/11/phospholipase C-independent, p38 mitogen-activated protein kinase-dependent activation of Na⁺/H⁺ exchanger

Tsunaki Higa, Takahiro Horinouchi, Hiroyuki Aoyagi, Hiroshi Asano, Tadashi Nishiya, Arata Nishimoto, Ikunobu Muramatsu, Soichi Miwa

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

The mechanism for sustained Ca²⁺ influx activated by G protein-coupled receptors was examined. In Chinese hamster ovary cells expressing recombinant human endothelin type B receptor (ET_BR) and endogenous P2Y receptor (P2Y-R), endothelin-1 elicited a sustained Ca²⁺ influx depending on G_q/11 protein, phospholipase C (PLC), Na⁺/H⁺ exchanger (NHE), and p38 mitogen-activated protein kinase (p38MAPK), whereas P2Y-R-induced sustained Ca²⁺ influx was negligible. Functional studies showed that NHE activation by ET_BR was mediated via p38MAPK but not G_q/11/PLC, while that by P2Y-R involves only G_q/11/PLC/p38MAPK. These results suggest that G_q/11/PLC-independent NHE activation via p38MAPK plays an important role in ET_BR-mediated sustained Ca²⁺ influx.

Original language	English
Pages (from-to)	276-280
Number of pages	5
Journal	Journal of Pharmacological Sciences
Volume	113
Issue number	3
DOIs	https://doi.org/10.1254/jphs.10102SC
Publication status	Published - 2010
Externally published	Yes

All Science Journal Classification (ASJC) codes

Molecular Medicine
Pharmacology

Access to Document

10.1254/jphs.10102SC

Cite this

Higa, T., Horinouchi, T., Aoyagi, H., Asano, H., Nishiya, T., Nishimoto, A., Muramatsu, I., & Miwa, S. (2010). Endothelin type B receptor-induced sustained Ca²⁺ influx involves G_q/11/phospholipase C-independent, p38 mitogen-activated protein kinase-dependent activation of Na⁺/H⁺ exchanger. Journal of Pharmacological Sciences, 113(3), 276-280. https://doi.org/10.1254/jphs.10102SC

Endothelin type B receptor-induced sustained Ca²⁺ influx involves G_q/11/phospholipase C-independent, p38 mitogen-activated protein kinase-dependent activation of Na⁺/H⁺ exchanger. / Higa, Tsunaki; Horinouchi, Takahiro; Aoyagi, Hiroyuki et al.
In: Journal of Pharmacological Sciences, Vol. 113, No. 3, 2010, p. 276-280.

Research output: Contribution to journal › Article › peer-review

Higa, T, Horinouchi, T, Aoyagi, H, Asano, H, Nishiya, T, Nishimoto, A, Muramatsu, I & Miwa, S 2010, 'Endothelin type B receptor-induced sustained Ca²⁺ influx involves G_q/11/phospholipase C-independent, p38 mitogen-activated protein kinase-dependent activation of Na⁺/H⁺ exchanger', Journal of Pharmacological Sciences, vol. 113, no. 3, pp. 276-280. https://doi.org/10.1254/jphs.10102SC

@article{73dfea13fe024cebbcbdac291bbd91c5,

title = "Endothelin type B receptor-induced sustained Ca2+ influx involves Gq/11/phospholipase C-independent, p38 mitogen-activated protein kinase-dependent activation of Na+/H+ exchanger",

abstract = "The mechanism for sustained Ca2+ influx activated by G protein-coupled receptors was examined. In Chinese hamster ovary cells expressing recombinant human endothelin type B receptor (ETBR) and endogenous P2Y receptor (P2Y-R), endothelin-1 elicited a sustained Ca2+ influx depending on Gq/11 protein, phospholipase C (PLC), Na+/H+ exchanger (NHE), and p38 mitogen-activated protein kinase (p38MAPK), whereas P2Y-R-induced sustained Ca2+ influx was negligible. Functional studies showed that NHE activation by ETBR was mediated via p38MAPK but not Gq/11/PLC, while that by P2Y-R involves only Gq/11/PLC/p38MAPK. These results suggest that Gq/11/PLC-independent NHE activation via p38MAPK plays an important role in ETBR-mediated sustained Ca2+ influx.",

author = "Tsunaki Higa and Takahiro Horinouchi and Hiroyuki Aoyagi and Hiroshi Asano and Tadashi Nishiya and Arata Nishimoto and Ikunobu Muramatsu and Soichi Miwa",

year = "2010",

doi = "10.1254/jphs.10102SC",

language = "English",

volume = "113",

pages = "276--280",

journal = "Journal of Pharmacological Sciences",

issn = "1347-8613",

publisher = "Japanese Pharmacological Society",

number = "3",

}

TY - JOUR

T1 - Endothelin type B receptor-induced sustained Ca2+ influx involves Gq/11/phospholipase C-independent, p38 mitogen-activated protein kinase-dependent activation of Na+/H+ exchanger

AU - Higa, Tsunaki

AU - Horinouchi, Takahiro

AU - Aoyagi, Hiroyuki

AU - Asano, Hiroshi

AU - Nishiya, Tadashi

AU - Nishimoto, Arata

AU - Muramatsu, Ikunobu

AU - Miwa, Soichi

PY - 2010

Y1 - 2010

N2 - The mechanism for sustained Ca2+ influx activated by G protein-coupled receptors was examined. In Chinese hamster ovary cells expressing recombinant human endothelin type B receptor (ETBR) and endogenous P2Y receptor (P2Y-R), endothelin-1 elicited a sustained Ca2+ influx depending on Gq/11 protein, phospholipase C (PLC), Na+/H+ exchanger (NHE), and p38 mitogen-activated protein kinase (p38MAPK), whereas P2Y-R-induced sustained Ca2+ influx was negligible. Functional studies showed that NHE activation by ETBR was mediated via p38MAPK but not Gq/11/PLC, while that by P2Y-R involves only Gq/11/PLC/p38MAPK. These results suggest that Gq/11/PLC-independent NHE activation via p38MAPK plays an important role in ETBR-mediated sustained Ca2+ influx.

AB - The mechanism for sustained Ca2+ influx activated by G protein-coupled receptors was examined. In Chinese hamster ovary cells expressing recombinant human endothelin type B receptor (ETBR) and endogenous P2Y receptor (P2Y-R), endothelin-1 elicited a sustained Ca2+ influx depending on Gq/11 protein, phospholipase C (PLC), Na+/H+ exchanger (NHE), and p38 mitogen-activated protein kinase (p38MAPK), whereas P2Y-R-induced sustained Ca2+ influx was negligible. Functional studies showed that NHE activation by ETBR was mediated via p38MAPK but not Gq/11/PLC, while that by P2Y-R involves only Gq/11/PLC/p38MAPK. These results suggest that Gq/11/PLC-independent NHE activation via p38MAPK plays an important role in ETBR-mediated sustained Ca2+ influx.

UR - http://www.scopus.com/inward/record.url?scp=77954980178&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77954980178&partnerID=8YFLogxK

U2 - 10.1254/jphs.10102SC

DO - 10.1254/jphs.10102SC

M3 - Article

C2 - 20595785

AN - SCOPUS:77954980178

SN - 1347-8613

VL - 113

SP - 276

EP - 280

JO - Journal of Pharmacological Sciences

JF - Journal of Pharmacological Sciences

IS - 3

ER -

Endothelin type B receptor-induced sustained Ca²⁺ influx involves G_q/11/phospholipase C-independent, p38 mitogen-activated protein kinase-dependent activation of Na⁺/H⁺ exchanger

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this