TY - JOUR
T1 - En bloc incorporation of coatomer subunits during the assembly of COP- coated vesicles
AU - Hara-Kuge, S.
AU - Kuge, O.
AU - Orci, L.
AU - Amherdt, M.
AU - Ravazzola, M.
AU - Wieland, F. T.
AU - Rothman, J. E.
PY - 1994
Y1 - 1994
N2 - The cDNA encoding ε-COP, the 36-kD subunit of coatomer, was cloned from a bovine liver cDNA library and sequenced. Immunoblotting with an anti-ε-COP antibody showed that ε-COP exists in COP-coated vesicles as well as in the cytosolic coatomer. Using the cloned cDNA, recombinant His6-tagged ε-COP was overexpressed in cultured Chinese hamster ovary (CHO) cells, from which metabolically radiolabeled coatomer was purified by taking advantage of the His6 tag. Radiolabeled coatomer was employed to establish that all the subunits of the coatomer enter coated vesicles as an intact unit.
AB - The cDNA encoding ε-COP, the 36-kD subunit of coatomer, was cloned from a bovine liver cDNA library and sequenced. Immunoblotting with an anti-ε-COP antibody showed that ε-COP exists in COP-coated vesicles as well as in the cytosolic coatomer. Using the cloned cDNA, recombinant His6-tagged ε-COP was overexpressed in cultured Chinese hamster ovary (CHO) cells, from which metabolically radiolabeled coatomer was purified by taking advantage of the His6 tag. Radiolabeled coatomer was employed to establish that all the subunits of the coatomer enter coated vesicles as an intact unit.
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U2 - 10.1083/jcb.124.6.883
DO - 10.1083/jcb.124.6.883
M3 - Article
C2 - 8132710
AN - SCOPUS:0028226938
SN - 0021-9525
VL - 124
SP - 883
EP - 892
JO - Journal of Cell Biology
JF - Journal of Cell Biology
IS - 6
ER -