Electrokinetic preconcentration and highly stacking gel electrophoresis for analysis of high molecular weight protein complexes on a plastic microchip

Mohamad Reza Mohamadi; Laili Mahmoudian; Noritada Kaji; Manabu Tokeshi; Hiroshi Chuman; Yoshinobu Baba

Electrokinetic preconcentration and highly stacking gel electrophoresis for analysis of high molecular weight protein complexes on a plastic microchip

Mohamad Reza Mohamadi, Laili Mahmoudian, Noritada Kaji, Manabu Tokeshi, Hiroshi Chuman, Yoshinobu Baba

Research output: Contribution to conference › Paper › peer-review

Abstract

An efficient dynamic coating using methylcellulose and a non-ionic detergent (Tween-20) was developed which controlled protein adsorption onto the surface of microchannels on a microchip made of poly (methyl methacrylate), PMMA. Highly stacking microchip electrophoresis under non-denaturing condition was used for analyzing high molecular weight protein complexes on a plastic microchip. The method has the efficiency of 6*10 6 plate/m for a complex of mAb-HSA with MW of ∼220kDa and with the detection limit of few pM.

Original language	English
Pages	353-355
Number of pages	3
Publication status	Published - Jan 1 2006
Externally published	Yes
Event	10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 - Tokyo, Japan Duration: Nov 5 2006 → Nov 9 2006

Other

Other	10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006
Country/Territory	Japan
City	Tokyo
Period	11/5/06 → 11/9/06

All Science Journal Classification (ASJC) codes

Chemical Engineering (miscellaneous)
Bioengineering

Cite this

Mohamadi, M. R., Mahmoudian, L., Kaji, N., Tokeshi, M., Chuman, H., & Baba, Y. (2006). Electrokinetic preconcentration and highly stacking gel electrophoresis for analysis of high molecular weight protein complexes on a plastic microchip. 353-355. Paper presented at 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006, Tokyo, Japan.

Electrokinetic preconcentration and highly stacking gel electrophoresis for analysis of high molecular weight protein complexes on a plastic microchip. / Mohamadi, Mohamad Reza; Mahmoudian, Laili; Kaji, Noritada et al.
2006. 353-355 Paper presented at 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006, Tokyo, Japan.

Research output: Contribution to conference › Paper › peer-review

Mohamadi, MR, Mahmoudian, L, Kaji, N, Tokeshi, M, Chuman, H & Baba, Y 2006, 'Electrokinetic preconcentration and highly stacking gel electrophoresis for analysis of high molecular weight protein complexes on a plastic microchip', Paper presented at 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006, Tokyo, Japan, 11/5/06 - 11/9/06 pp. 353-355.

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AU - Mohamadi, Mohamad Reza

AU - Mahmoudian, Laili

AU - Kaji, Noritada

AU - Tokeshi, Manabu

AU - Chuman, Hiroshi

AU - Baba, Yoshinobu

PY - 2006/1/1

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N2 - An efficient dynamic coating using methylcellulose and a non-ionic detergent (Tween-20) was developed which controlled protein adsorption onto the surface of microchannels on a microchip made of poly (methyl methacrylate), PMMA. Highly stacking microchip electrophoresis under non-denaturing condition was used for analyzing high molecular weight protein complexes on a plastic microchip. The method has the efficiency of 6*10 6 plate/m for a complex of mAb-HSA with MW of ∼220kDa and with the detection limit of few pM.

AB - An efficient dynamic coating using methylcellulose and a non-ionic detergent (Tween-20) was developed which controlled protein adsorption onto the surface of microchannels on a microchip made of poly (methyl methacrylate), PMMA. Highly stacking microchip electrophoresis under non-denaturing condition was used for analyzing high molecular weight protein complexes on a plastic microchip. The method has the efficiency of 6*10 6 plate/m for a complex of mAb-HSA with MW of ∼220kDa and with the detection limit of few pM.

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T2 - 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006

Y2 - 5 November 2006 through 9 November 2006

ER -

Electrokinetic preconcentration and highly stacking gel electrophoresis for analysis of high molecular weight protein complexes on a plastic microchip

Abstract

Other

All Science Journal Classification (ASJC) codes

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