TY - JOUR
T1 - Electrochemical assay for saccharide-protein interactions using glycopolymer-modified gold nanoparticles
AU - Ishii, Jin
AU - Chikae, Miyuki
AU - Toyoshima, Masayuki
AU - Ukita, Yoshiaki
AU - Miura, Yoshiko
AU - Takamura, Yuzuru
N1 - Funding Information:
This work was supported by a Grant-in-Aid for Scientific Research on Innovative Areas ( 20106003 ), and by the Program for Developing the Supporting System for Upgrading the Education and Research .
PY - 2011/8
Y1 - 2011/8
N2 - A novel recognition element, made of electroactive glycopolymer-modified gold nanoparticles (GM-GNPs), was synthesized and used in an electrochemical assay of saccharide-protein interactions for the detection of concanavalin A (Con A). The electroactivity of the mannose-based GM-GNPs was investigated, and an electrochemical Con A assay system with a disposable screen printed carbon strips was proposed. A complex containing GM-GNPs was formed by a recognition reaction between the mannose-based glycopolymer and Con A. The complex was detected by the pre-oxidation of the GNPs and following reduction scans using differential pulse voltammetry (DPV). Under optimal conditions, a linear relationship between the DPV peak current intensity and Con A concentration was shown within an analytical range of 10-10,000 ng/mL. Our proposed new electric approach provides an interesting tool for the analysis of saccharide-protein interactions.
AB - A novel recognition element, made of electroactive glycopolymer-modified gold nanoparticles (GM-GNPs), was synthesized and used in an electrochemical assay of saccharide-protein interactions for the detection of concanavalin A (Con A). The electroactivity of the mannose-based GM-GNPs was investigated, and an electrochemical Con A assay system with a disposable screen printed carbon strips was proposed. A complex containing GM-GNPs was formed by a recognition reaction between the mannose-based glycopolymer and Con A. The complex was detected by the pre-oxidation of the GNPs and following reduction scans using differential pulse voltammetry (DPV). Under optimal conditions, a linear relationship between the DPV peak current intensity and Con A concentration was shown within an analytical range of 10-10,000 ng/mL. Our proposed new electric approach provides an interesting tool for the analysis of saccharide-protein interactions.
UR - http://www.scopus.com/inward/record.url?scp=79960744127&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79960744127&partnerID=8YFLogxK
U2 - 10.1016/j.elecom.2011.05.014
DO - 10.1016/j.elecom.2011.05.014
M3 - Article
AN - SCOPUS:79960744127
SN - 1388-2481
VL - 13
SP - 830
EP - 833
JO - Electrochemistry Communications
JF - Electrochemistry Communications
IS - 8
ER -