TY - JOUR
T1 - Efficient protein expression in Bombyx mori larvae of the strain d17 highly sensitive to B. mori nucleopolyhedrovirus
AU - Kawakami, Naoya
AU - Lee, Jae Man
AU - Mon, Hiroaki
AU - Kubo, Yuji
AU - Banno, Yutaka
AU - Kawaguchi, Yutaka
AU - Maenaka, Katsumi
AU - Park, Enoch Y.
AU - Koga, Katsumi
AU - Kusakabe, Takahiro
N1 - Funding Information:
Acknowledgements This work was supported in part by the Program for Promotion of Basic Research Activities for Innovative Biosciences (PROBRAIN), and by a grants-in-aid no. 17380037 from the Ministry of Education, Science and Culture of Japan.
PY - 2008/10
Y1 - 2008/10
N2 - The recombinant protein expression by Bombyx mori nucleopolyhedrovirus (BmNPV) infecting silkworm larvae or pupae may endow us with a potent system for the production of large eukaryotic proteins. However, the screening of silkworm strains ideally suited to this method has scarcely been conducted. In the present study, we injected recombinant BmNPV containing a reporter gene, luciferase or DsRed, into hemocoel of fifth instar larvae of selected 12 silkworm strains. Among them, the strain d17 is found to be the highest in reporter expression from the intrinsic polyhedrin promoter of Autographa californica NPV or the silkworm actin A3 promoter. These results suggest that the d17 strain is highly permissive for BmNPV replication and is the most likely candidate of a "factory" for large-scale expression using the BmNPV bacmid system.
AB - The recombinant protein expression by Bombyx mori nucleopolyhedrovirus (BmNPV) infecting silkworm larvae or pupae may endow us with a potent system for the production of large eukaryotic proteins. However, the screening of silkworm strains ideally suited to this method has scarcely been conducted. In the present study, we injected recombinant BmNPV containing a reporter gene, luciferase or DsRed, into hemocoel of fifth instar larvae of selected 12 silkworm strains. Among them, the strain d17 is found to be the highest in reporter expression from the intrinsic polyhedrin promoter of Autographa californica NPV or the silkworm actin A3 promoter. These results suggest that the d17 strain is highly permissive for BmNPV replication and is the most likely candidate of a "factory" for large-scale expression using the BmNPV bacmid system.
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U2 - 10.1007/s12033-008-9074-3
DO - 10.1007/s12033-008-9074-3
M3 - Article
C2 - 18543128
AN - SCOPUS:56749153928
SN - 1073-6085
VL - 40
SP - 180
EP - 185
JO - Molecular Biotechnology
JF - Molecular Biotechnology
IS - 2
ER -