Efficient production of recombinant human erythropoietin by replenishment of microcarriers in the hollow fiber culture cassette

Yuichi Inoue; Nobuhisa Arita; Kiichiro Teruya; Yoshinori Katakura; Sanetaka Shirahata

doi:10.1271/bbb.63.1624

Efficient production of recombinant human erythropoietin by replenishment of microcarriers in the hollow fiber culture cassette

Yuichi Inoue, Nobuhisa Arita, Kiichiro Teruya, Yoshinori Katakura, Sanetaka Shirahata

Systems Biology

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Human erythropoietin (EPO)-producing recombinant BHK cells were cultured in culture medium containing microcarriers, and then microcarriers attached with cells were replenished in the hollow fiber culture cassette. By culture for 14 days, it was possible to produce 450 μg of the recombinant EPO, which corresponded to over two-fold of the recombinant EPO production by control hollow fiber culture without microcarriers.

Original language	English
Pages (from-to)	1624-1626
Number of pages	3
Journal	Bioscience, Biotechnology and Biochemistry
Volume	63
Issue number	9
DOIs	https://doi.org/10.1271/bbb.63.1624
Publication status	Published - Jan 1 1999

All Science Journal Classification (ASJC) codes

Medicine(all)

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abstract = "Human erythropoietin (EPO)-producing recombinant BHK cells were cultured in culture medium containing microcarriers, and then microcarriers attached with cells were replenished in the hollow fiber culture cassette. By culture for 14 days, it was possible to produce 450 μg of the recombinant EPO, which corresponded to over two-fold of the recombinant EPO production by control hollow fiber culture without microcarriers.",

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AU - Arita, Nobuhisa

AU - Teruya, Kiichiro

AU - Katakura, Yoshinori

AU - Shirahata, Sanetaka

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Efficient production of recombinant human erythropoietin by replenishment of microcarriers in the hollow fiber culture cassette

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