TY - JOUR
T1 - Efficient delivery of signal-responsive gene carriers for disease-specific gene expression via bubble liposomes and sonoporation
AU - Tsuchiya, Akira
AU - Kang, Jeong Hun
AU - Mori, Takeshi
AU - Naritomi, Yuki
AU - Kushio, Satoshi
AU - Niidome, Takuro
AU - Tachibana, Katsuro
AU - Takahashi, Yoko
AU - Negishi, Yoichi
AU - Oda, Yusuke
AU - Suzuki, Ryo
AU - Maruyama, Kazuo
AU - Katayama, Yoshiki
N1 - Funding Information:
This work was financially supported by a Grant-in-Aid for Scientific Research from the Japanese Ministry of Education, Culture, Sports, Science and Technology of Japan ( 16K11010 ).
Publisher Copyright:
© 2017 Elsevier B.V.
PY - 2017/12/1
Y1 - 2017/12/1
N2 - Sonoporation is a promising method to intracellularly deliver synthetic gene carriers that have lower endocytotic uptake than viral carriers. Here, we applied sonoporation to deliver genes via polyethylene glycol (PEG)-grafted polymeric carriers that specifically respond to hyperactivated protein kinase A (PKA). PEG-grafted polymeric carrier/DNA polyplexes were not efficiently delivered into cells via the endocytotic pathway because of the hydrophilic PEG layer surrounding the polyplexes. However, the delivery of polyplexes into cells was significantly increased by sonoporation. The delivered polyplexes exhibited PKA-responsive transgene expression in PKA-overexpressing cells, but not in cells with low PKA activation. These results show that the sonoporation-mediated delivery of PEG-modified PKA-responsive polyplexes is a promising approach for safely applying gene therapy to abnormal cells with hyperactivated PKA.
AB - Sonoporation is a promising method to intracellularly deliver synthetic gene carriers that have lower endocytotic uptake than viral carriers. Here, we applied sonoporation to deliver genes via polyethylene glycol (PEG)-grafted polymeric carriers that specifically respond to hyperactivated protein kinase A (PKA). PEG-grafted polymeric carrier/DNA polyplexes were not efficiently delivered into cells via the endocytotic pathway because of the hydrophilic PEG layer surrounding the polyplexes. However, the delivery of polyplexes into cells was significantly increased by sonoporation. The delivered polyplexes exhibited PKA-responsive transgene expression in PKA-overexpressing cells, but not in cells with low PKA activation. These results show that the sonoporation-mediated delivery of PEG-modified PKA-responsive polyplexes is a promising approach for safely applying gene therapy to abnormal cells with hyperactivated PKA.
UR - http://www.scopus.com/inward/record.url?scp=85029282036&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85029282036&partnerID=8YFLogxK
U2 - 10.1016/j.colsurfb.2017.09.021
DO - 10.1016/j.colsurfb.2017.09.021
M3 - Article
C2 - 28917150
AN - SCOPUS:85029282036
SN - 0927-7765
VL - 160
SP - 60
EP - 64
JO - Colloids and Surfaces B: Biointerfaces
JF - Colloids and Surfaces B: Biointerfaces
ER -