TY - JOUR
T1 - Efficient cross-linking reactions by functional nucleobases capable of in situ activation under neutral conditions.
AU - Nagatsugi, Fumi
AU - Suenaga, Yasushi
AU - Sasaki, Shigeki
PY - 2003
Y1 - 2003
N2 - We have previously described that the oligonucleotides containing phenylsulfide derivatives of 2-amino-6-vinylpurine nucleoside analog are activated within duplex to form cross-link toward cytidine selectively at the target site. However, the cross-linking reaction via the in-situ activation needs acidic conditions. In this paper, we wish to report that the new 2-amino-6-vinylpurine derivatives designed to have a structure of "double-activation" for in-situ activation exhibit fast cross-linking with cytidine under neutral conditions.
AB - We have previously described that the oligonucleotides containing phenylsulfide derivatives of 2-amino-6-vinylpurine nucleoside analog are activated within duplex to form cross-link toward cytidine selectively at the target site. However, the cross-linking reaction via the in-situ activation needs acidic conditions. In this paper, we wish to report that the new 2-amino-6-vinylpurine derivatives designed to have a structure of "double-activation" for in-situ activation exhibit fast cross-linking with cytidine under neutral conditions.
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U2 - 10.1093/nass/3.1.155
DO - 10.1093/nass/3.1.155
M3 - Article
C2 - 14510427
AN - SCOPUS:0142212290
SP - 155
EP - 156
JO - Nucleic acids research. Supplement (2001)
JF - Nucleic acids research. Supplement (2001)
IS - 3
ER -