Effects of concurrent exposure to tributyltin and 1,1-dichloro-2,2 bis (p-chlorophenyl) ethylene (p,p′-DDE) on immature male Wistar rats

Yuji Makita, Minoru Omura, Akiyo Tanaka, Chikako Kiyohara

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23 Citations (Scopus)


Tributyltin and 1, 1-dichloro-2, 2 bis (p-chlorophenyl) ethylene (p,p′-DDE) have been ubiquitously distributed over the world. In Japan, p,p′-DDE and tributyltin are ingested through marine products, in which these substances are accumulated through bio-concentration and the food chain. However, the consequence of potential combined hazards of these substances remains unknown. Therefore, the effects of concurrent exposure to 125 ppm p,p′-DDE and 25 ppm tributyltin were investigated in immature male Wistar rats by oral administration during puberty. In this study, tributyltin promoted the growth of pubertal male rats, while p,p′-DDE itself did not affect the growth but inhibited the growth enhancement by tributyltin. Furthermore, tributyltin reduced thymus weight but p,p′-DDE also prevented this weight reduction. Neither development of male sexual accessory organs nor sexual maturation was affected even by concurrent exposure to p,p′-DDE and tributyltin. No significant changes of serum testosterone, luteinizing hormone, follicle-stimulating hormone concentrations, and epididymal sperm numbers were observed with the administration of p,p′-DDE and/or tributyltin. These results indicate that sexual maturation, male reproductive organ development and sperm production is scarcely affected in immature male Wistar rats even by concurrent exposure to p,p′-DDE and tributyltin at a daily dose of ca. 2 mg/kg tributyltin and 10 mg/kg p,p′-DDE. Moreover, the simultaneous administration of p,p′-DDE with tributyltin counterbalanced the effects that were attributed to tributyltin alone.

Original languageEnglish
Pages (from-to)364-368
Number of pages5
JournalBasic and Clinical Pharmacology and Toxicology
Issue number6
Publication statusPublished - Dec 2005

All Science Journal Classification (ASJC) codes

  • Toxicology
  • Pharmacology


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