Effects of atmospheric-pressure non-thermal plasma jets on enzyme solutions

Pankaj Attri; Pannuru Venkatesu; Nagendra Kaushik; Yong Gyu Han; Chul Joo Nam; Eun Ha Choi; Key Sun Kim

doi:10.3938/jkps.60.959

Effects of atmospheric-pressure non-thermal plasma jets on enzyme solutions

Pankaj Attri, Pannuru Venkatesu, Nagendra Kaushik, Yong Gyu Han, Chul Joo Nam, Eun Ha Choi, Key Sun Kim

Research output: Contribution to journal › Article › peer-review

24 Citations (Scopus)

Abstract

In order to study the wide scope of structural information of biomolecules in the presence of biocompatible cold atmospheric pressure plasma jets (CAPJ), we used circular dichroism (CD) and fluorescence experiments. Biomolecules are very sensitive and are highly complex systems, exhibiting a substantial degree of structural variability in their folded state. In the present investigation, we compared the compatiblity of α-chymotrypsin (CT) in the presence of biocompatible CAPJ. Using circular dichroism (CD) and fluorescence experiments, we describe the associated structural changes in CT after a 5 min treatment with CAPJ. The modifications in the secondary structure of this β/β protein were quantified by using the CD spectra. A reasonable decrease was observed for β-strands after treatment with CAPJ as compared to buffer, which contributes to its deterioration power. The implications of our results from studies delineating the effects of CAPJ on enzyme solutions are discussed.

Original language	English
Pages (from-to)	959-964
Number of pages	6
Journal	Journal of the Korean Physical Society
Volume	60
Issue number	6
DOIs	https://doi.org/10.3938/jkps.60.959
Publication status	Published - Mar 2012
Externally published	Yes

All Science Journal Classification (ASJC) codes

Physics and Astronomy(all)

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title = "Effects of atmospheric-pressure non-thermal plasma jets on enzyme solutions",

abstract = "In order to study the wide scope of structural information of biomolecules in the presence of biocompatible cold atmospheric pressure plasma jets (CAPJ), we used circular dichroism (CD) and fluorescence experiments. Biomolecules are very sensitive and are highly complex systems, exhibiting a substantial degree of structural variability in their folded state. In the present investigation, we compared the compatiblity of α-chymotrypsin (CT) in the presence of biocompatible CAPJ. Using circular dichroism (CD) and fluorescence experiments, we describe the associated structural changes in CT after a 5 min treatment with CAPJ. The modifications in the secondary structure of this β/β protein were quantified by using the CD spectra. A reasonable decrease was observed for β-strands after treatment with CAPJ as compared to buffer, which contributes to its deterioration power. The implications of our results from studies delineating the effects of CAPJ on enzyme solutions are discussed.",

author = "Pankaj Attri and Pannuru Venkatesu and Nagendra Kaushik and Han, {Yong Gyu} and Nam, {Chul Joo} and Choi, {Eun Ha} and Kim, {Key Sun}",

note = "Funding Information: We gratefully acknowledge the Science Research Center (SRC) program of the National Research Foundation of Korea (NRF), grant funded by the Ministry of Education, Science and Technology (MEST), Korean Government (MEST, No. 20100029418). This research is supported in part by Kwangwoon University 2011. PA gratefully acknowledge to the Plasma Bioscience Research Center (PBRC) for providing laboratory and financial assistance for research and PV{\textquoteright}s thankful to Council of Scientific Industrial Research (CSIR), New Delhi, for grant No. 01(2343)/09/EMR-II.",

year = "2012",

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doi = "10.3938/jkps.60.959",

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AU - Attri, Pankaj

AU - Venkatesu, Pannuru

AU - Kaushik, Nagendra

AU - Han, Yong Gyu

AU - Nam, Chul Joo

AU - Choi, Eun Ha

AU - Kim, Key Sun

N1 - Funding Information: We gratefully acknowledge the Science Research Center (SRC) program of the National Research Foundation of Korea (NRF), grant funded by the Ministry of Education, Science and Technology (MEST), Korean Government (MEST, No. 20100029418). This research is supported in part by Kwangwoon University 2011. PA gratefully acknowledge to the Plasma Bioscience Research Center (PBRC) for providing laboratory and financial assistance for research and PV’s thankful to Council of Scientific Industrial Research (CSIR), New Delhi, for grant No. 01(2343)/09/EMR-II.

PY - 2012/3

Y1 - 2012/3

N2 - In order to study the wide scope of structural information of biomolecules in the presence of biocompatible cold atmospheric pressure plasma jets (CAPJ), we used circular dichroism (CD) and fluorescence experiments. Biomolecules are very sensitive and are highly complex systems, exhibiting a substantial degree of structural variability in their folded state. In the present investigation, we compared the compatiblity of α-chymotrypsin (CT) in the presence of biocompatible CAPJ. Using circular dichroism (CD) and fluorescence experiments, we describe the associated structural changes in CT after a 5 min treatment with CAPJ. The modifications in the secondary structure of this β/β protein were quantified by using the CD spectra. A reasonable decrease was observed for β-strands after treatment with CAPJ as compared to buffer, which contributes to its deterioration power. The implications of our results from studies delineating the effects of CAPJ on enzyme solutions are discussed.

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Effects of atmospheric-pressure non-thermal plasma jets on enzyme solutions

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