TY - JOUR
T1 - Effects of apatite cements on proliferation and differentiation of human osteoblasts in vitro
AU - Yuasa, Tetsuya
AU - Miyamoto, Youji
AU - Ishikawa, Kunio
AU - Takechi, Masaaki
AU - Momota, Yukihiro
AU - Tatehara, Seiko
AU - Nagayama, Masaru
N1 - Funding Information:
This work was supported, in part by a grant-in-aid for Science Research from the Ministry of Education, Culture, Sports, Science and Technology, Japan.
PY - 2004
Y1 - 2004
N2 - Although apatite cement (AC) and sintered hydroxyapatite (s-HAP) are known to show good osteoconductivity, it is not clear whether or not the degree of their osteoconductivity is the same. In addition, it has not been clarified whether or not it is dependent on the type of AC; conventional AC (c-AC), fast-setting AC (fs-AC) or anti-washout AC (aw-AC). The aim of this study was to investigate the effects of ACs on cultured human osteoblasts, as they may provide a useful index of osteoconductivity. Human osteoblasts were cultured on the surfaces of ACs and s-HAP, and were evaluated with respect to cell attachment, proliferation, and differentiation. We found that ACs and s-HAP showed similar cell attachment. No significant difference between ACs and s-HAP was found with respect to the proliferation of osteoblasts. In contrast, we found that the differentiation of osteoblasts was enhanced on the surface of ACs compared with that of s-HAP. However, there was no difference among the types of AC. We therefore concluded that AC may show better osteoconductivity than s-HAP, and that osteoconductivity of AC may be similar, regardless of the type of AC.
AB - Although apatite cement (AC) and sintered hydroxyapatite (s-HAP) are known to show good osteoconductivity, it is not clear whether or not the degree of their osteoconductivity is the same. In addition, it has not been clarified whether or not it is dependent on the type of AC; conventional AC (c-AC), fast-setting AC (fs-AC) or anti-washout AC (aw-AC). The aim of this study was to investigate the effects of ACs on cultured human osteoblasts, as they may provide a useful index of osteoconductivity. Human osteoblasts were cultured on the surfaces of ACs and s-HAP, and were evaluated with respect to cell attachment, proliferation, and differentiation. We found that ACs and s-HAP showed similar cell attachment. No significant difference between ACs and s-HAP was found with respect to the proliferation of osteoblasts. In contrast, we found that the differentiation of osteoblasts was enhanced on the surface of ACs compared with that of s-HAP. However, there was no difference among the types of AC. We therefore concluded that AC may show better osteoconductivity than s-HAP, and that osteoconductivity of AC may be similar, regardless of the type of AC.
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U2 - 10.1016/j.biomaterials.2003.08.003
DO - 10.1016/j.biomaterials.2003.08.003
M3 - Article
C2 - 14643589
AN - SCOPUS:0344825280
SN - 0142-9612
VL - 25
SP - 1159
EP - 1166
JO - Biomaterials
JF - Biomaterials
IS - 7-8
ER -