Effect of titanium surface calcium and magnesium on adhesive activity of epithelial-like cells and fibroblasts

Hideyuki Okawachi, Yasunori Ayukawa, Ikiru Atsuta, Akihiro Furuhashi, Mami Sakaguchi, Koichi Yamane, Kiyoshi Koyano

Research output: Contribution to journalArticlepeer-review

39 Citations (Scopus)


In the present study, we investigated the hydrothermal treatment of titanium with divalent cation solutions and its effect in promoting the adhesion of gingival epithelial cells and fibroblasts in vitro. Gingival keratinocyte-like Sa3 cells or fibroblastic NIH3T3 cells were cultured for 1 h on experimental titanium plates hydrothermally-treated with CaCl2 (Ca) or MgCl2 (Mg) solution, or distilled water (DW). The number and adhesive strengths of attached cells on the substrata were then analyzed. The number of Sa3 cells adhering to the Ca- and Mg-treated plates was significantly larger than in the DW group, but the strength of this adhesion did not differ significantly between groups. In contrast, NIH3T3 cell adhesion number and strength were increased in both the Ca and Mg groups compared to the DW group. Fluorescent microscopic observation indicated that, in all groups, Sa3 had identical expression levels of integrin β4 and development of actin filaments, whereas NIH3T3 cells in the Ca and Mg groups displayed much stronger punctate cytoplasmic signals for vinculin and more bundle-shaped actin filaments than cells in the DW group. As a result, it was indicated that the hydrothermal treatment of titanium with Ca or Mg solution improved the integration of soft tissue cells with the substrata, which may facilitate the development of a soft tissue barrier around the implant.

Original languageEnglish
Article number27
Pages (from-to)1-8
Number of pages8
Issue number1-4
Publication statusPublished - 2012

All Science Journal Classification (ASJC) codes

  • General Chemistry
  • Biomaterials
  • General Materials Science
  • General Biochemistry,Genetics and Molecular Biology
  • General Physics and Astronomy


Dive into the research topics of 'Effect of titanium surface calcium and magnesium on adhesive activity of epithelial-like cells and fibroblasts'. Together they form a unique fingerprint.

Cite this