TY - JOUR
T1 - Effect of temperature on the inhibition of the GABA-gated response by intracellular calcium
AU - Maruyama, Tooru
AU - Ikemoto, Yoshimi
AU - Akaike, Norio
N1 - Funding Information:
Ad, nowledgements We thank Dr k S Kits tor advice in preparing the manuscript This research was supported by Grants-m-A~d to N A from the Japanese Ministry of Education Scmnce and (ulture Nos 62870102 6348(111)7 6~641~26 and 01641'~30
PY - 1990/1/15
Y1 - 1990/1/15
N2 - The effects of the voltage-dependent Ca2+inward current (ICa) on γ-aminobutyric acid (GABA)-induced Cl- current (ICl) in isolated sensory neurons were examined at different temperatures and with different concentrations of external Ca2+ using the 'concentration-clamp' technique. The total amount of inhibition of GABA-induced ICl by a preceding ICa increased in a hyperbolic manner with increasing Ca2+ influx. The time course of recovery of the GABA response after inhibition by Ca 2+influx followed a single exponential and was facilitated by warming but slowed dramatically by a slight cooling from 20 to 15 °C in spite of a decrease in Ca2+ influx. It is discussed that the energy-dependent, temperature-sensitive ionic pump and exchange systems at the surface membrane and intracellular organelles regulate the cytoplasmic free Ca2+, thus explaining the quantitative effects of Ca2+ influx and temperature on the inhibition of the GABA-gated Cl- channel.
AB - The effects of the voltage-dependent Ca2+inward current (ICa) on γ-aminobutyric acid (GABA)-induced Cl- current (ICl) in isolated sensory neurons were examined at different temperatures and with different concentrations of external Ca2+ using the 'concentration-clamp' technique. The total amount of inhibition of GABA-induced ICl by a preceding ICa increased in a hyperbolic manner with increasing Ca2+ influx. The time course of recovery of the GABA response after inhibition by Ca 2+influx followed a single exponential and was facilitated by warming but slowed dramatically by a slight cooling from 20 to 15 °C in spite of a decrease in Ca2+ influx. It is discussed that the energy-dependent, temperature-sensitive ionic pump and exchange systems at the surface membrane and intracellular organelles regulate the cytoplasmic free Ca2+, thus explaining the quantitative effects of Ca2+ influx and temperature on the inhibition of the GABA-gated Cl- channel.
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U2 - 10.1016/0006-8993(90)90515-D
DO - 10.1016/0006-8993(90)90515-D
M3 - Article
C2 - 2302574
AN - SCOPUS:0025124599
SN - 0006-8993
VL - 507
SP - 17
EP - 22
JO - Brain Research
JF - Brain Research
IS - 1
ER -