Effect of poly(L-lysine)-g-dextran copolymers on DNA hybridization.

Longliang Wu; Naohiko Shimada; Arihiro Kano; Atsushi Maruyama

doi:10.1093/nass/nrm037

Effect of poly(L-lysine)-g-dextran copolymers on DNA hybridization.

Longliang Wu, Naohiko Shimada, Arihiro Kano, Atsushi Maruyama

Department of Fundamental Organic Chemistry

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

We have focused on design of biomaterials that are capable of engineering nucleic acids folding. In this study, we explored the effect of poly(L-lysine)-g-dextran, PLL-g-Dex, copolymer on hybridization kinetics of DNA. The hybridization kinetics was studied by using a fluorescence resonance energy transfer (FRET) assay in the absence and presence of the copolymer. The copolymer at nano molar concentration significantly accelerated the hybridization rate over 200-fold under physiologically relevant ionic conditions.

Original language	English
Pages (from-to)	73-74
Number of pages	2
Journal	Nucleic acids symposium series (2004)
Issue number	51
DOIs	https://doi.org/10.1093/nass/nrm037
Publication status	Published - 2007

All Science Journal Classification (ASJC) codes

Medicine(all)

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10.1093/nass/nrm037

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title = "Effect of poly(L-lysine)-g-dextran copolymers on DNA hybridization.",

abstract = "We have focused on design of biomaterials that are capable of engineering nucleic acids folding. In this study, we explored the effect of poly(L-lysine)-g-dextran, PLL-g-Dex, copolymer on hybridization kinetics of DNA. The hybridization kinetics was studied by using a fluorescence resonance energy transfer (FRET) assay in the absence and presence of the copolymer. The copolymer at nano molar concentration significantly accelerated the hybridization rate over 200-fold under physiologically relevant ionic conditions.",

author = "Longliang Wu and Naohiko Shimada and Arihiro Kano and Atsushi Maruyama",

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AU - Shimada, Naohiko

AU - Kano, Arihiro

AU - Maruyama, Atsushi

PY - 2007

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AB - We have focused on design of biomaterials that are capable of engineering nucleic acids folding. In this study, we explored the effect of poly(L-lysine)-g-dextran, PLL-g-Dex, copolymer on hybridization kinetics of DNA. The hybridization kinetics was studied by using a fluorescence resonance energy transfer (FRET) assay in the absence and presence of the copolymer. The copolymer at nano molar concentration significantly accelerated the hybridization rate over 200-fold under physiologically relevant ionic conditions.

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JF - Nucleic acids symposium series (2004)

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Effect of poly(L-lysine)-g-dextran copolymers on DNA hybridization.

Abstract

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