Effect of organic solvents on extraction behaviour of cytochrome c using reversed micellar solutions

Tsutomu Ono, Takayuki Miyazaki, Masahiro Goto, Fumiyuki Nakashio

    Research output: Contribution to journalArticlepeer-review

    8 Citations (Scopus)

    Abstract

    The effect of organic solvents on the extraction behaviour of cytochrome c has been investigated with reversed micellar solutions. An aliphatic solvent such as hexane, octane or isooctane was suitable for protein extraction operations using the reversed micelles. Organic solvents having a different hydrophobicity show significantly different behaviour in protein extraction, where extraction efficiency appears to be governed by the nature of the interfacial complex that is formed by surfactants and proteins. Solvent effects on the protein extraction are more dominant in the AOT system rather than that in the DOLPA system. Addition of alcohol appears to decrease the activity of proteins in the back-extraction step, although it is effective for recovering proteins from the reversed micellar solutions.

    Original languageEnglish
    Pages (from-to)1-9
    Number of pages9
    JournalSolvent Extraction Research and Development, Japan
    Volume1996
    Issue number3
    Publication statusPublished - 1996

    All Science Journal Classification (ASJC) codes

    • General Chemistry
    • General Chemical Engineering
    • Filtration and Separation

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