Effect of nanosecond-pulsed plasma on the structural modification of biomolecules

Ji Hoon Park, Naresh Kumar, Han Sup Uhm, Weontae Lee, Eun Ha Choi, Pankaj Attri

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13 Citations (Scopus)


In this study, we generated reactive species (RS) (OH, H, NO, H2O2, ONOO-, ONOOH, O2-, HO2, NO2-) through the use of nanosecond-pulsed plasma (NPP). In order, to understand the action of NPP on biomolecules, we have studied circular dichroism (CD) spectroscopy (spectral and thermodynamic analysis), gel electrophoresis, 1D NMR, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and Liquid Chromatograph/Capillary Electrophoresis-Mass Spectrometer (LC/CE-MS) for quantitative and qualitative bioanalysis. The measurements revealed that the RS generated by the NPP structurally modified the proteins [myoglobin (Mb) and hemoglobin (Hb)], amino acids [lysine (Lys), l-proline, l-threonine (Thr), l-asparagine (Asn), l-glutamic acid (Glu) and glycine] and calf thymus DNA. After the discharge, the thermodynamic profile of Hb exhibited more changes compared to Mb. Furthermore, we examined the protein carbonylation/oxidation to understand the structural modification for both proteins after NPP treatment. Moreover, we also observed the oxidation in calf thymus DNA after the NPP treatment. As a result, these studies extend the scope for next generation plasma-based treatments and understanding of the mechanism of NPP on biomolecules.

Original languageEnglish
Pages (from-to)47300-47308
Number of pages9
JournalRSC Advances
Issue number59
Publication statusPublished - 2015
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Chemical Engineering(all)


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