TY - JOUR
T1 - Effect of MTAD on the Differentiation of Osteoblast-like Cells
AU - Yasuda, Yoshiyuki
AU - Tatematsu, Yuki
AU - Fujii, Shinsuke
AU - Maeda, Hidefumi
AU - Akamine, Akifumi
AU - Torabinejad, Mahmoud
AU - Saito, Takashi
N1 - Funding Information:
This work was supported by Grant-in-Aid for Scientific Research (nos. 18659563 and 18791407 ) from the Japan Society for the Promotion of Science , and by a grant from the Research Center, Health Sciences University of Hokkaido .
PY - 2010/2
Y1 - 2010/2
N2 - Introduction: The aim of the present study was to investigate the effect of MTAD on the differentiation of osteoblast-like cells. Methods: The cell viability assay was performed to evaluate the cytotoxicity of MTAD on MC3T3-E1 and periodontal ligament cells at the various concentrations. The bone sialoprotein (BSP) gene expression was also examined by real-time polymerase chain reaction. Results: MTAD exhibited a lower cytotoxicity compared with other intracanal irrigants and medication. The MC3T3-E1 cells treated with H 2O 2 showed a decrease in the alkaline phosphatase (ALP) activity by 40% on day 14 compared with the control group at the concentration of 50 μg/mlL. No significant difference in the ALP activity was observed between MTAD and control group. Furthermore, MTAD and Ca(OH) 2 paste did not change in BSP gene expression in MC3T3-E1 cells on day 21. Conclusions: These results suggested that MTAD is a less cytotoxic irrigant and does not affect differentiation into osteoblasts compared with other intracanal irrigants, such as H 2O 2, NaOCl, ethylenediaminetetraacetic acid, and chlorhexidine.
AB - Introduction: The aim of the present study was to investigate the effect of MTAD on the differentiation of osteoblast-like cells. Methods: The cell viability assay was performed to evaluate the cytotoxicity of MTAD on MC3T3-E1 and periodontal ligament cells at the various concentrations. The bone sialoprotein (BSP) gene expression was also examined by real-time polymerase chain reaction. Results: MTAD exhibited a lower cytotoxicity compared with other intracanal irrigants and medication. The MC3T3-E1 cells treated with H 2O 2 showed a decrease in the alkaline phosphatase (ALP) activity by 40% on day 14 compared with the control group at the concentration of 50 μg/mlL. No significant difference in the ALP activity was observed between MTAD and control group. Furthermore, MTAD and Ca(OH) 2 paste did not change in BSP gene expression in MC3T3-E1 cells on day 21. Conclusions: These results suggested that MTAD is a less cytotoxic irrigant and does not affect differentiation into osteoblasts compared with other intracanal irrigants, such as H 2O 2, NaOCl, ethylenediaminetetraacetic acid, and chlorhexidine.
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U2 - 10.1016/j.joen.2009.11.002
DO - 10.1016/j.joen.2009.11.002
M3 - Article
C2 - 20113785
AN - SCOPUS:74549189113
SN - 0099-2399
VL - 36
SP - 260
EP - 263
JO - Journal of Endodontics
JF - Journal of Endodontics
IS - 2
ER -