TY - JOUR
T1 - Effect of highly lipolyzed goat cheese on HL-60 human leukemia cells
T2 - Antiproliferative activity and induction of apoptotic DNA damage
AU - Yasuda, S.
AU - Kuwata, H.
AU - Kawamoto, K.
AU - Shirakawa, J.
AU - Atobe, S.
AU - Hoshi, Y.
AU - Yamasaki, M.
AU - Nishiyama, K.
AU - Tachibana, H.
AU - Yamada, K.
AU - Kobayashi, H.
AU - Igoshi, K.
N1 - Funding Information:
The authors thank Daijiro Hirayama and Yukari Takatsuka (School of Agriculture, Tokai University) for technical assistance. This work was supported in part by grant from Japan Dairy Association (Tokyo, Japan) and by Research and Study Program/Project of Tokai University Educational System General Research Organization (Kanagawa, Japan).
PY - 2012/5
Y1 - 2012/5
N2 - To establish cheese as a dairy product with health benefits, we embarked on examining the multifunctional role of cheeses, especially in the field of cancer prevention. The current study was designed to investigate whether different types of commercial goat cheeses may possess antiproliferative activity, using an HL-60 human promyelocytic leukemia cell line as a cancer cell model. Among 11 cheese extracts tested at 500μg/mL, 6 (Crottin de Chavignol, Pouligny Saint-Pierre, Chabichou du Poitou, Valencay, Kavli, and Sainte-Maure de Touraine) resulted in a significant decrease of cell viability, which is consistent with a decrease in viable cell number. Compared with the half-maximal inhibitory concentration (IC50) value of individual cheeses in cellular proliferation assays, the Pouligny Saint-Pierre extract showed strong inhibition. Incubation of cells in the presence of Pouligny Saint-Pierre extract resulted in induction of cellular morphological changes and apoptotic DNA fragmentation as well as expression of the active form of caspase-3 protein. Based on the quantification of the ratio of free fatty acids to triglycerides in different cheese samples, a significant correlation was detected between lipolytic ripeness and IC50 values for antiproliferative capacity tested in HL-60 cells. Collectively, these results support a potential role of highly lipolyzed goat cheeses in the prevention of leukemic cell proliferation.
AB - To establish cheese as a dairy product with health benefits, we embarked on examining the multifunctional role of cheeses, especially in the field of cancer prevention. The current study was designed to investigate whether different types of commercial goat cheeses may possess antiproliferative activity, using an HL-60 human promyelocytic leukemia cell line as a cancer cell model. Among 11 cheese extracts tested at 500μg/mL, 6 (Crottin de Chavignol, Pouligny Saint-Pierre, Chabichou du Poitou, Valencay, Kavli, and Sainte-Maure de Touraine) resulted in a significant decrease of cell viability, which is consistent with a decrease in viable cell number. Compared with the half-maximal inhibitory concentration (IC50) value of individual cheeses in cellular proliferation assays, the Pouligny Saint-Pierre extract showed strong inhibition. Incubation of cells in the presence of Pouligny Saint-Pierre extract resulted in induction of cellular morphological changes and apoptotic DNA fragmentation as well as expression of the active form of caspase-3 protein. Based on the quantification of the ratio of free fatty acids to triglycerides in different cheese samples, a significant correlation was detected between lipolytic ripeness and IC50 values for antiproliferative capacity tested in HL-60 cells. Collectively, these results support a potential role of highly lipolyzed goat cheeses in the prevention of leukemic cell proliferation.
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U2 - 10.3168/jds.2011-4153
DO - 10.3168/jds.2011-4153
M3 - Article
C2 - 22541454
AN - SCOPUS:84860255639
SN - 0022-0302
VL - 95
SP - 2248
EP - 2260
JO - Journal of Dairy Science
JF - Journal of Dairy Science
IS - 5
ER -
