TY - GEN
T1 - Effect of gap-junctional cell-to-cell communication on intracellular ice propagation and consequent cell viability
AU - Fukunaga, Takanobu
AU - Kubo, Hiroto
AU - Kurata, Kosaku
AU - Hirahara, Hideto
AU - Wang, Hai Dong
AU - Takamatsu, Hiroshi
PY - 2015
Y1 - 2015
N2 - Intracellular ice formation (IIF) propagates from cell to cell and causes lethal damage to the cell during the freezing process. In this study, we hypothesized that IIF passes through gap junctions between cells and that blockage of gap junctions increases cell viability after the freeze-thaw process because of the inhibited IIF propagation. Monolayer cultures of Madin-Darby canine kidney (MDCK) cells on glass coverslips were preincubated with and without the gap-junction blocker heptanol and subsequently frozen at different cooling rates on a temperature-controlled stage. Microscopic observations showed that IIF propagation was successfully inhibited in the blocked group. However, cell viability in the blocked group after thawing was significantly lower than that in the non-blocked group. Although results of this study did not confirm our hypotheses, they indicated the important role of gap-junctional communication in the occurrence of IIF and the consequent cell damage.
AB - Intracellular ice formation (IIF) propagates from cell to cell and causes lethal damage to the cell during the freezing process. In this study, we hypothesized that IIF passes through gap junctions between cells and that blockage of gap junctions increases cell viability after the freeze-thaw process because of the inhibited IIF propagation. Monolayer cultures of Madin-Darby canine kidney (MDCK) cells on glass coverslips were preincubated with and without the gap-junction blocker heptanol and subsequently frozen at different cooling rates on a temperature-controlled stage. Microscopic observations showed that IIF propagation was successfully inhibited in the blocked group. However, cell viability in the blocked group after thawing was significantly lower than that in the non-blocked group. Although results of this study did not confirm our hypotheses, they indicated the important role of gap-junctional communication in the occurrence of IIF and the consequent cell damage.
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U2 - 10.18462/iir.icr.2015.0437
DO - 10.18462/iir.icr.2015.0437
M3 - Conference contribution
AN - SCOPUS:85016813915
T3 - Refrigeration Science and Technology
SP - 4521
EP - 4527
BT - 24th IIR International Congress of Refrigeration, ICR 2015
PB - International Institute of Refrigeration
T2 - 24th IIR International Congress of Refrigeration, ICR 2015
Y2 - 16 August 2015 through 22 August 2015
ER -