Effect of DNA methylation on the velocity of DNA translocation through a nanochannel

Xiaoyin Sun, Takao Yasui, Takeshi Yanagida, Noritada Kaji, Sakon Rahong, Masaki Kanai, Kazuki Nagashima, Tomoji Kawai, Yoshinobu Baba

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Here, we report the effect of DNA methylation on the velocity of DNA translocation through a nanochannel, as determined by measuring differences in translocation velocities between methylated and non-methylated DNA molecules. We found that the velocity of translocation of methylated DNA was faster than that of non-methylated DNA, which we attributed to variation in the coefficients of diffusion and friction with the nanochannel wall, due to the increased molecular weight and stiffness, respectively, of methylated DNA.

Original languageEnglish
Pages (from-to)727-730
Number of pages4
Journalanalytical sciences
Volume33
Issue number6
DOIs
Publication statusPublished - 2017

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

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