Effect of diazinon exposure on antioxidant reactions in the silkmoth, Bombyx mori

Kohji Yamamoto; Y. Tsuji; Y. Aso; T. Hamasaki; S. Shirahata; Y. Katakura

doi:10.1111/j.1439-0418.2010.01549.x

Effect of diazinon exposure on antioxidant reactions in the silkmoth, Bombyx mori

Kohji Yamamoto, Y. Tsuji, Y. Aso, T. Hamasaki, S. Shirahata, Y. Katakura

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

We investigated the effects of exposure to diazinon, an organophosphate insecticide, on the expression of antioxidant and detoxification factors in the silkmoth. Exposure to diazinon resulted in induction of mRNA encoding manganese containing superoxide dismutase (SOD) and omega-class glutathione S-transferases (GST), whereas no changes were observed in catalase, other class of GST and acetylcholinesterase. Liquid chromatography showed that the amount of reactive oxygen species was increased, whereas the amount of glutathione was decreased in the silkmoth fat body after exposure to diazinon. These results suggest that SOD and omega-class GSTs can contribute to organophosphate resistance in Lepidopterans.

Original language	English
Pages (from-to)	320-325
Number of pages	6
Journal	Journal of Applied Entomology
Volume	135
Issue number	4
DOIs	https://doi.org/10.1111/j.1439-0418.2010.01549.x
Publication status	Published - May 2011

All Science Journal Classification (ASJC) codes

Agronomy and Crop Science
Insect Science

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abstract = "We investigated the effects of exposure to diazinon, an organophosphate insecticide, on the expression of antioxidant and detoxification factors in the silkmoth. Exposure to diazinon resulted in induction of mRNA encoding manganese containing superoxide dismutase (SOD) and omega-class glutathione S-transferases (GST), whereas no changes were observed in catalase, other class of GST and acetylcholinesterase. Liquid chromatography showed that the amount of reactive oxygen species was increased, whereas the amount of glutathione was decreased in the silkmoth fat body after exposure to diazinon. These results suggest that SOD and omega-class GSTs can contribute to organophosphate resistance in Lepidopterans.",

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AU - Yamamoto, Kohji

AU - Tsuji, Y.

AU - Aso, Y.

AU - Hamasaki, T.

AU - Shirahata, S.

AU - Katakura, Y.

PY - 2011/5

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N2 - We investigated the effects of exposure to diazinon, an organophosphate insecticide, on the expression of antioxidant and detoxification factors in the silkmoth. Exposure to diazinon resulted in induction of mRNA encoding manganese containing superoxide dismutase (SOD) and omega-class glutathione S-transferases (GST), whereas no changes were observed in catalase, other class of GST and acetylcholinesterase. Liquid chromatography showed that the amount of reactive oxygen species was increased, whereas the amount of glutathione was decreased in the silkmoth fat body after exposure to diazinon. These results suggest that SOD and omega-class GSTs can contribute to organophosphate resistance in Lepidopterans.

AB - We investigated the effects of exposure to diazinon, an organophosphate insecticide, on the expression of antioxidant and detoxification factors in the silkmoth. Exposure to diazinon resulted in induction of mRNA encoding manganese containing superoxide dismutase (SOD) and omega-class glutathione S-transferases (GST), whereas no changes were observed in catalase, other class of GST and acetylcholinesterase. Liquid chromatography showed that the amount of reactive oxygen species was increased, whereas the amount of glutathione was decreased in the silkmoth fat body after exposure to diazinon. These results suggest that SOD and omega-class GSTs can contribute to organophosphate resistance in Lepidopterans.

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Effect of diazinon exposure on antioxidant reactions in the silkmoth, Bombyx mori

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