Effect of Azo Dyes on Proliferation in Mouse Embryo Culture Cells

Yoshiko Okawa, Makoto Yoshimoto, Takahisa Miyamoto, Shoji Hatano

Research output: Contribution to journalArticlepeer-review


Amaranth (Food Red No. 2) and Ponceau 3R stimulate in vitro RNA synthesis by causing the dissociation of chromatin in isolated rat-liver nuclei. The effects of these azo dyes and Sunset Yellow FCF (Food Yellow No. 5) on proliferation of mouse embryo culture cells, C3H10 T1/2, were studied. 1) When the cells were pretreated for 1 hr with S9-untreated azo dyes at concentrations of 0.3. 1.0, and 5 mM, the cell growth was slightly reduced by 5 mM Ponceau 3R. All the S9 -untreated azo dyes inhibited cell growth when added to the medium during cultivation. 2) The 1-hr pretreatment of the cells with S9-treated amaranth and Ponceau 3R at a concentration of 0.3 mM temporarily accelerated the cell growth in the early stage of cell proliferation. However, this was not observed when these S9-treated azo dyes were added to the medium during cultivation.

Original languageEnglish
Pages (from-to)496
Number of pages1
JournalFood Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi)
Issue number6
Publication statusPublished - 1989

All Science Journal Classification (ASJC) codes

  • Food Science
  • Public Health, Environmental and Occupational Health


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