TY - JOUR
T1 - Effect of AVT antisense oligodeoxynucleotides on AVT release induced by hypertonic stimulation in chicks
AU - Saito, Noboru
AU - Sasaki, Takeshi
AU - Furuse, Mitsuhiro
AU - Arakawa, Koji
AU - Shimada, Kiyoshi
N1 - Funding Information:
We thank Dr D.A. Fisher and Dr T.I. Koike for providing AVT and MT antibodies, respectively. We also appreciate the Radioisotope Research Center of Nagoya University for use of the facilities. This study was supported in part by Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan (09660318 and 11460124) to N. Saito and M. Furuse, respectively.
PY - 2001
Y1 - 2001
N2 - In birds, arginine vasotocin (AVT) and mesotocin (MT) are the neurohypophyseal hormones. AVT is known to be an avian antidiuretic hormone and is released from the neurohypophysis by dehydration or hyperosmotic stimulation. The purpose of this study was to determine whether the mechanism of AVT synthesis is related to the mechanism of hormone release from the neurohypophysis. Four-day-old chicks received an AVT antisense oligodeoxynucleotide (ODN) injection into the cerebral ventricle (icv). Following antisense administration, the chicks received hypertonic saline stimulation. Plasma levels of AVT and MT were measured by radioimmunoassays. In control birds, a hypertonic saline injection resulted in the increase of plasma AVT level. The administration of a high dose (50 μg) of antisense ODN inhibited the increase of plasma AVT level induced by the hypertonic saline stimulation. Plasma levels of MT did not change with the administration of hypertonic saline or antisense ODN. These results suggest that the mechanisms that regulate the secretion of AVT from the neurohypophysis may be coupled to the mechanisms that regulate the synthesis of AVT.
AB - In birds, arginine vasotocin (AVT) and mesotocin (MT) are the neurohypophyseal hormones. AVT is known to be an avian antidiuretic hormone and is released from the neurohypophysis by dehydration or hyperosmotic stimulation. The purpose of this study was to determine whether the mechanism of AVT synthesis is related to the mechanism of hormone release from the neurohypophysis. Four-day-old chicks received an AVT antisense oligodeoxynucleotide (ODN) injection into the cerebral ventricle (icv). Following antisense administration, the chicks received hypertonic saline stimulation. Plasma levels of AVT and MT were measured by radioimmunoassays. In control birds, a hypertonic saline injection resulted in the increase of plasma AVT level. The administration of a high dose (50 μg) of antisense ODN inhibited the increase of plasma AVT level induced by the hypertonic saline stimulation. Plasma levels of MT did not change with the administration of hypertonic saline or antisense ODN. These results suggest that the mechanisms that regulate the secretion of AVT from the neurohypophysis may be coupled to the mechanisms that regulate the synthesis of AVT.
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U2 - 10.1016/S1095-6433(00)00290-7
DO - 10.1016/S1095-6433(00)00290-7
M3 - Article
C2 - 11137447
AN - SCOPUS:0035181268
SN - 1095-6433
VL - 128
SP - 147
EP - 153
JO - Comparative Biochemistry and Physiology - A Molecular and Integrative Physiology
JF - Comparative Biochemistry and Physiology - A Molecular and Integrative Physiology
IS - 1
M1 - 6478
ER -