Effect of apatite cements on human osteoblasts in vitro

T. Yuasa, Y. Miyamoto, K. Ishikawa, M. Takechi, Y. Momota, T. Toh, M. Nagayama, K. Suzuki

Research output: Contribution to journalConference articlepeer-review

1 Citation (Scopus)


A conventional apatite cement (c-AC) used for reconstruction of bony defect takes 30 min to set and decays upon exposure to liquid before setting. Therefore, we have developed two types of new ACs; fast-setting AC and anti-washout type AC. The aims of this study are to investigate the effect of ACs on cultured osteoblasts and to compare effects of new ACs on osteoblasts with that of c-AC. Disc shape set ACs with almost same surface roughness were prepared and evaluated by powder X-ray diffraction (XRD). The clonal human osteoblasts were cultured on each specimens. Plastic dish and sintered hydroxyapatite (HAP) were used as control. ACs showed the same apatitic XRD patterns with poor crystallinity when compared with sintered HAP. There were no differences in proliferation of osteoblasts among ACs and sintered HAP. The alkaline phosphatase activity and protein levels for type I collagen and osteocalcin of ACs were greater than those of sintered HAP. There was no difference among ACs. In conclusion, it is suggested that ACs could promote the differentiation of osteoblasts as compared with sintered HAP and plastic. Also, it is suggested that two types of new ACs would show the same cell response as c-AC in vivo.

Original languageEnglish
Pages (from-to)845-848
Number of pages4
JournalKey Engineering Materials
Publication statusPublished - 2001
Externally publishedYes
Event13th international Symposium on Ceramics in Medicine (BIOCERAMICS) - Bologna, Italy
Duration: Nov 22 2000Nov 26 2000

All Science Journal Classification (ASJC) codes

  • General Materials Science
  • Mechanics of Materials
  • Mechanical Engineering


Dive into the research topics of 'Effect of apatite cements on human osteoblasts in vitro'. Together they form a unique fingerprint.

Cite this