TY - JOUR
T1 - Dynamic modification of poly(methyl methacrylate) chips using poly(vinyl alcohol) for glycosaminoglycan disaccharide isomer separation
AU - Zhang, Yong
AU - Ping, Guichen
AU - Kaji, Noritada
AU - Tokeshi, Manabu
AU - Baba, Yoshi
PY - 2007/9
Y1 - 2007/9
N2 - We describe a microchip electrophoresis (MCE) method for the assay of unsaturated disaccharides of chondroitin sulfates, dermatan sulfates, and hyaluronic acid (HA). Poly(vinyl alcohol) (PVA) could be irreversibly adsorbed onto poly(methyl methacrylate) (PMMA) substrates and this approach was applicable for dynamic coating. The characteristics of the PMMA surface with PVA coating were evaluated in terms of the wettability, EOF, and adsorption of 2-aminoacridone (AMAC)-labeled disaccharide. The water contact angle decreased from ∼73° on a pristine PMMA surface to ∼37.5° on a PVA-coated surface, indicating that the PVA coating increased hydrophilicity. EOF was reduced approximately twofold and was relatively stable. Scanning electron microscopy and fluorescence microscopy images showed that adsorption of AMAC-labeled disaccharides was dramatically suppressed. Using the PVA coating, baseline separation of two pairs of glycosaminoglycan (GAG) disaccharide isomers, ΔDi-diSB/ΔDi-diSD and ΔDi-0S/ΔDi-HA, was achieved in Tris-borate buffer within 130 s by MCE.
AB - We describe a microchip electrophoresis (MCE) method for the assay of unsaturated disaccharides of chondroitin sulfates, dermatan sulfates, and hyaluronic acid (HA). Poly(vinyl alcohol) (PVA) could be irreversibly adsorbed onto poly(methyl methacrylate) (PMMA) substrates and this approach was applicable for dynamic coating. The characteristics of the PMMA surface with PVA coating were evaluated in terms of the wettability, EOF, and adsorption of 2-aminoacridone (AMAC)-labeled disaccharide. The water contact angle decreased from ∼73° on a pristine PMMA surface to ∼37.5° on a PVA-coated surface, indicating that the PVA coating increased hydrophilicity. EOF was reduced approximately twofold and was relatively stable. Scanning electron microscopy and fluorescence microscopy images showed that adsorption of AMAC-labeled disaccharides was dramatically suppressed. Using the PVA coating, baseline separation of two pairs of glycosaminoglycan (GAG) disaccharide isomers, ΔDi-diSB/ΔDi-diSD and ΔDi-0S/ΔDi-HA, was achieved in Tris-borate buffer within 130 s by MCE.
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U2 - 10.1002/elps.200700088
DO - 10.1002/elps.200700088
M3 - Article
C2 - 17722188
AN - SCOPUS:34848909340
SN - 0173-0835
VL - 28
SP - 3308
EP - 3314
JO - ELECTROPHORESIS
JF - ELECTROPHORESIS
IS - 18
ER -