The cryogel obtained from the patient with rheumatoid arthritis composes of fibronectin (FN), fibrinogen and heparin. In addition, a large quantity of EDA(+)FN, one of the cellular FN, was included. In order to identify the mechanism of cryogel formation, we attempt to determine the hydrodynamic radius (Rh) of the aggregates consist of FN and heparin by dynamic light scattering method using DLS-700 (Otsuka electronics, JPN) with Argon-ion laser (488 nm) as the light source. We found that the Rh of EDA(+)FN increase with addition of heparin, in contrast to the fact that plasma FN do not aggregate with heparin. We also found that the Rh of the EDA(+)FN and heparin aggregates is hundred times larger than that of plasma FN at body temperature region and ten times at lower temperature region. Thus we conclude that the EDA(+)FN is the nucleus materials for the cryogel formation and cooling of the plasma makes the cryofiltration effective and easier.
|Number of pages
|Japanese Journal of Artificial Organs
|Published - 1995
All Science Journal Classification (ASJC) codes