Dynamic light scattering study of cryogel formation

K. Miyamoto, T. Nakamura, Y. Shimonishi, M. Tokita, T. Komai, M. Yonekawa, A. Kawamura, N. Kobayashi, K. Miyashita, E. Sakashita

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1 Citation (Scopus)


The cryogel obtained from the patient with rheumatoid arthritis composes of fibronectin (FN), fibrinogen and heparin. In addition, a large quantity of EDA(+)FN, one of the cellular FN, was included. In order to identify the mechanism of cryogel formation, we attempt to determine the hydrodynamic radius (Rh) of the aggregates consist of FN and heparin by dynamic light scattering method using DLS-700 (Otsuka electronics, JPN) with Argon-ion laser (488 nm) as the light source. We found that the Rh of EDA(+)FN increase with addition of heparin, in contrast to the fact that plasma FN do not aggregate with heparin. We also found that the Rh of the EDA(+)FN and heparin aggregates is hundred times larger than that of plasma FN at body temperature region and ten times at lower temperature region. Thus we conclude that the EDA(+)FN is the nucleus materials for the cryogel formation and cooling of the plasma makes the cryofiltration effective and easier.

Original languageEnglish
Pages (from-to)70-73
Number of pages4
JournalJapanese Journal of Artificial Organs
Issue number1
Publication statusPublished - 1995
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics


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