One-dimensional gratings with different duty ratios were designed and implemented for enhanced fluorescence detection and imaging. Verified by finite difference time domain simulations, our results showed that the enhancement strongly depended on the duty ratio of the land width to pitch of the grating structure. The maximum enhancement factor was achieved when the duty ratio was equal to 0.50 in our trapezoidal gratings with pitch=400 nm and depth=20 nm. Such a facile grating mold will exert a considerable influence on microarray biosensors and fluorescence microscopy.
All Science Journal Classification (ASJC) codes
- Physics and Astronomy (miscellaneous)