Dominant expression of interleukin 10 but not interferon γ in CD4-CD8- αβT cells of autoimmune lymphoproliferative syndrome

Shouichi Ohga; Akihiko Nomura; Yasushi Takahata; Kenji Ihara; Hidetoshi Takada; Hiroshi Wakiguchi; Yuji Kudo; Toshiro Hara

doi:10.1046/j.1365-2141.2002.03848.x

Dominant expression of interleukin 10 but not interferon γ in CD4^-CD8^- αβT cells of autoimmune lymphoproliferative syndrome

Shouichi Ohga, Akihiko Nomura, Yasushi Takahata, Kenji Ihara, Hidetoshi Takada, Hiroshi Wakiguchi, Yuji Kudo, Toshiro Hara

Reproductive and Developmental Medicine

Research output: Contribution to journal › Article › peer-review

25 Citations (Scopus)

Abstract

Cytokine expression in CD4^-CD8^- double-negative (DN) T cells of autoimmune lymphoproliferative syndrome (ALPS) was analysed. Two patients with DN αβT-cell expansion showed higher serum interleukin 10 (IL-10) levels than one patient without it. Intracellular flow-cytometric analysis indicated the IL-10-expressing CD3⁺CD4^-CD8^- cells in patients with lymphoproliferation. Quantitative real-time polymerase chain reaction revealed ∼100 times higher IL-10, but not interferon-γ or transforming growth factor-β in DN than in single-positive T cells. IL-10 was exclusively expressed in DN αβ but not γδT cells. Circulating DN αβT cells may constitutively express IL-10 and contribute to the ALPS phenotype.

Original language	English
Pages (from-to)	535-538
Number of pages	4
Journal	British Journal of Haematology
Volume	119
Issue number	2
DOIs	https://doi.org/10.1046/j.1365-2141.2002.03848.x
Publication status	Published - 2002

All Science Journal Classification (ASJC) codes

Hematology

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10.1046/j.1365-2141.2002.03848.x

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title = "Dominant expression of interleukin 10 but not interferon γ in CD4-CD8- αβT cells of autoimmune lymphoproliferative syndrome",

abstract = "Cytokine expression in CD4-CD8- double-negative (DN) T cells of autoimmune lymphoproliferative syndrome (ALPS) was analysed. Two patients with DN αβT-cell expansion showed higher serum interleukin 10 (IL-10) levels than one patient without it. Intracellular flow-cytometric analysis indicated the IL-10-expressing CD3+CD4-CD8- cells in patients with lymphoproliferation. Quantitative real-time polymerase chain reaction revealed ∼100 times higher IL-10, but not interferon-γ or transforming growth factor-β in DN than in single-positive T cells. IL-10 was exclusively expressed in DN αβ but not γδT cells. Circulating DN αβT cells may constitutively express IL-10 and contribute to the ALPS phenotype.",

author = "Shouichi Ohga and Akihiko Nomura and Yasushi Takahata and Kenji Ihara and Hidetoshi Takada and Hiroshi Wakiguchi and Yuji Kudo and Toshiro Hara",

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doi = "10.1046/j.1365-2141.2002.03848.x",

language = "English",

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T1 - Dominant expression of interleukin 10 but not interferon γ in CD4-CD8- αβT cells of autoimmune lymphoproliferative syndrome

AU - Ohga, Shouichi

AU - Nomura, Akihiko

AU - Takahata, Yasushi

AU - Ihara, Kenji

AU - Takada, Hidetoshi

AU - Wakiguchi, Hiroshi

AU - Kudo, Yuji

AU - Hara, Toshiro

PY - 2002

Y1 - 2002

N2 - Cytokine expression in CD4-CD8- double-negative (DN) T cells of autoimmune lymphoproliferative syndrome (ALPS) was analysed. Two patients with DN αβT-cell expansion showed higher serum interleukin 10 (IL-10) levels than one patient without it. Intracellular flow-cytometric analysis indicated the IL-10-expressing CD3+CD4-CD8- cells in patients with lymphoproliferation. Quantitative real-time polymerase chain reaction revealed ∼100 times higher IL-10, but not interferon-γ or transforming growth factor-β in DN than in single-positive T cells. IL-10 was exclusively expressed in DN αβ but not γδT cells. Circulating DN αβT cells may constitutively express IL-10 and contribute to the ALPS phenotype.

AB - Cytokine expression in CD4-CD8- double-negative (DN) T cells of autoimmune lymphoproliferative syndrome (ALPS) was analysed. Two patients with DN αβT-cell expansion showed higher serum interleukin 10 (IL-10) levels than one patient without it. Intracellular flow-cytometric analysis indicated the IL-10-expressing CD3+CD4-CD8- cells in patients with lymphoproliferation. Quantitative real-time polymerase chain reaction revealed ∼100 times higher IL-10, but not interferon-γ or transforming growth factor-β in DN than in single-positive T cells. IL-10 was exclusively expressed in DN αβ but not γδT cells. Circulating DN αβT cells may constitutively express IL-10 and contribute to the ALPS phenotype.

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Dominant expression of interleukin 10 but not interferon γ in CD4^-CD8^- αβT cells of autoimmune lymphoproliferative syndrome

Abstract

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