TY - JOUR
T1 - Domesticating Vigna Stipulacea
T2 - A Potential Legume Crop With Broad Resistance to Biotic Stresses
AU - Takahashi, Yu
AU - Sakai, Hiroaki
AU - Yoshitsu, Yuki
AU - Muto, Chiaki
AU - Anai, Toyoaki
AU - Pandiyan, Muthaiyan
AU - Senthil, Natesan
AU - Tomooka, Norihiko
AU - Naito, Ken
N1 - Funding Information:
We are grateful for Ms. Motoyoshi, Ms. Asano and Ms. Yamamoto for taking good care of plant materials. We also appreciate for the support provided by Research Supporting Program of the Advanced Analysis Center, National Agriculture and Food Research Organization (NARO) and the Genebank Project, NARO.
Funding Information:
This study was supported by JSPS KAKENHI Grant Number 13J09808, 19KT0016, and 26850006. It was also partially supported by Research Supporting Program of the Advanced Analysis Center, National Agriculture and Food Research Organization (NARO) and the Genebank Project, NARO.
Publisher Copyright:
© Copyright © 2019 Takahashi, Sakai, Yoshitsu, Muto, Anai, Pandiyan, Senthil, Tomooka and Naito.
PY - 2019/12/6
Y1 - 2019/12/6
N2 - Though crossing wild relatives to modern cultivars is a usual means to introduce alleles of stress tolerance, an alternative is de novo domesticating wild species that are already tolerant to various kinds of stresses. As a test case, we chose Vigna stipulacea Kuntze, which has fast growth, short vegetative stage, and broad resistance to pests and diseases. We developed an ethyl methanesulfonate–mutagenized population and obtained three mutants with reduced seed dormancy and one with reduced pod shattering. We crossed one of the mutants of less seed dormancy to the wild type and confirmed that the phenotype was inherited in a Mendelian manner. De novo assembly of V. stipulacea genome, and the following resequencing of the F2 progenies successfully identified a Single Nucleotide Polymorphism (SNP) associated with seed dormancy. By crossing and pyramiding the mutant phenotypes, we will be able to turn V. stipulacea into a crop which is yet primitive but can be cultivated without pesticides.
AB - Though crossing wild relatives to modern cultivars is a usual means to introduce alleles of stress tolerance, an alternative is de novo domesticating wild species that are already tolerant to various kinds of stresses. As a test case, we chose Vigna stipulacea Kuntze, which has fast growth, short vegetative stage, and broad resistance to pests and diseases. We developed an ethyl methanesulfonate–mutagenized population and obtained three mutants with reduced seed dormancy and one with reduced pod shattering. We crossed one of the mutants of less seed dormancy to the wild type and confirmed that the phenotype was inherited in a Mendelian manner. De novo assembly of V. stipulacea genome, and the following resequencing of the F2 progenies successfully identified a Single Nucleotide Polymorphism (SNP) associated with seed dormancy. By crossing and pyramiding the mutant phenotypes, we will be able to turn V. stipulacea into a crop which is yet primitive but can be cultivated without pesticides.
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U2 - 10.3389/fpls.2019.01607
DO - 10.3389/fpls.2019.01607
M3 - Article
AN - SCOPUS:85077257020
SN - 1664-462X
VL - 10
JO - Frontiers in Plant Science
JF - Frontiers in Plant Science
M1 - 1607
ER -