Do pluripotent stem cells exist in adult mice as very small embryonic stem cells?

Masanori Miyanishi; Yasuo Mori; Jun Seita; James Y. Chen; Seth Karten; Charles K.F. Chan; Hiromitsu Nakauchi; Irving L. Weissman

doi:10.1016/j.stemcr.2013.07.001

Do pluripotent stem cells exist in adult mice as very small embryonic stem cells?

Masanori Miyanishi, Yasuo Mori, Jun Seita, James Y. Chen, Seth Karten, Charles K.F. Chan, Hiromitsu Nakauchi, Irving L. Weissman

Research output: Contribution to journal › Article › peer-review

68 Citations (Scopus)

Abstract

Very small embryonic-like stem cells (VSELs) isolated from bone marrow (BM) have been reported to be pluripotent. Given their nonembryonic source, they could replace blastocyst-derived embryonic stem cells in research and medicine. However, their multiple-germ-layer potential has been incompletely studied. Here, we show that we cannot find VSELs in mouse BM with any of the reported stem cell potentials, specifically for hematopoiesis. We found that: (1) most events within the "VSEL" flow-cytometry gate had little DNA and the cells corresponding to these events (2) could not form spheres, (3) did not express Oct4, and (4) could not differentiate into blood cells. These results provide a failure to confirm the existence of pluripotent VSELs.

Original language	English
Pages (from-to)	198-208
Number of pages	11
Journal	Stem Cell Reports
Volume	1
Issue number	2
DOIs	https://doi.org/10.1016/j.stemcr.2013.07.001
Publication status	Published - 2013
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biochemistry
Genetics
Developmental Biology
Cell Biology

Access to Document

10.1016/j.stemcr.2013.07.001

Cite this

@article{339fad7923344ff89b2db44141ccad72,

title = "Do pluripotent stem cells exist in adult mice as very small embryonic stem cells?",

abstract = "Very small embryonic-like stem cells (VSELs) isolated from bone marrow (BM) have been reported to be pluripotent. Given their nonembryonic source, they could replace blastocyst-derived embryonic stem cells in research and medicine. However, their multiple-germ-layer potential has been incompletely studied. Here, we show that we cannot find VSELs in mouse BM with any of the reported stem cell potentials, specifically for hematopoiesis. We found that: (1) most events within the {"}VSEL{"} flow-cytometry gate had little DNA and the cells corresponding to these events (2) could not form spheres, (3) did not express Oct4, and (4) could not differentiate into blood cells. These results provide a failure to confirm the existence of pluripotent VSELs.",

author = "Masanori Miyanishi and Yasuo Mori and Jun Seita and Chen, {James Y.} and Seth Karten and Chan, {Charles K.F.} and Hiromitsu Nakauchi and Weissman, {Irving L.}",

note = "Funding Information: This study was supported by fellowships from the Toyobo Biotechnology Foundation, the Uehara Memorial Foundation, Human Frontier Science Program (to M.M.), and the Japan Society for the Promotion of Science (to Y.M.) and by grants from the US National Institutes of Health (R01 CA086065 and U01 HL099999 to I.L.W.), the California Institute for Regenerative Medicine (RT2-02060 to I.L.W.), and the Leukemia & Lymphoma Society (700709 to I.L.W.). H.N. is a shareholder and a member of the scientific advisory board of ReproCELL and Megakaryon and a member of the scientific advisory board of Shionogi. I.L.W. is a member of the scientific advisory board of StemCells. ",

year = "2013",

doi = "10.1016/j.stemcr.2013.07.001",

language = "English",

volume = "1",

pages = "198--208",

journal = "Stem Cell Reports",

issn = "2213-6711",