DNA stretching method using a flow induced by laser irradiation under AC electric field

Michihiko Nakano; Kazunori Takashima; Shinji Katsura; Akira Mizuno

DNA stretching method using a flow induced by laser irradiation under AC electric field

Michihiko Nakano, Kazunori Takashima, Shinji Katsura, Akira Mizuno

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

A micro-vortex is generated around an infrared laser focus where an intense AC electric field is applied. We used this opto-electrostatic micro-vortex to stretch individual long DNA molecules. When T4-phage DNA molecules were introduced into the opto-electrostatic micro-vortex, they were stretched around the laser focus. In addition, it was possible to keep it in stretching form for more than 30 sec. Using this method, length of DNA molecules can be measured without fixing to a substrate.

Original language	English
Title of host publication	Micro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Publisher	Japan Academic Association Inc
Pages	1525-1527
Number of pages	3
Publication status	Published - 2006
Externally published	Yes
Event	10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 - Tokyo, Japan Duration: Nov 5 2006 → Nov 9 2006

Other

Other	10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006
Country/Territory	Japan
City	Tokyo
Period	11/5/06 → 11/9/06

All Science Journal Classification (ASJC) codes

Chemical Engineering (miscellaneous)
Bioengineering

Cite this

DNA stretching method using a flow induced by laser irradiation under AC electric field. / Nakano, Michihiko; Takashima, Kazunori; Katsura, Shinji et al.
Micro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Japan Academic Association Inc, 2006. p. 1525-1527.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Nakano, M, Takashima, K, Katsura, S & Mizuno, A 2006, DNA stretching method using a flow induced by laser irradiation under AC electric field. in Micro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Japan Academic Association Inc, pp. 1525-1527, 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006, Tokyo, Japan, 11/5/06.

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abstract = "A micro-vortex is generated around an infrared laser focus where an intense AC electric field is applied. We used this opto-electrostatic micro-vortex to stretch individual long DNA molecules. When T4-phage DNA molecules were introduced into the opto-electrostatic micro-vortex, they were stretched around the laser focus. In addition, it was possible to keep it in stretching form for more than 30 sec. Using this method, length of DNA molecules can be measured without fixing to a substrate.",

author = "Michihiko Nakano and Kazunori Takashima and Shinji Katsura and Akira Mizuno",

year = "2006",

language = "English",

pages = "1525--1527",

booktitle = "Micro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences",

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note = "10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 ; Conference date: 05-11-2006 Through 09-11-2006",

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AU - Nakano, Michihiko

AU - Takashima, Kazunori

AU - Katsura, Shinji

AU - Mizuno, Akira

PY - 2006

Y1 - 2006

N2 - A micro-vortex is generated around an infrared laser focus where an intense AC electric field is applied. We used this opto-electrostatic micro-vortex to stretch individual long DNA molecules. When T4-phage DNA molecules were introduced into the opto-electrostatic micro-vortex, they were stretched around the laser focus. In addition, it was possible to keep it in stretching form for more than 30 sec. Using this method, length of DNA molecules can be measured without fixing to a substrate.

AB - A micro-vortex is generated around an infrared laser focus where an intense AC electric field is applied. We used this opto-electrostatic micro-vortex to stretch individual long DNA molecules. When T4-phage DNA molecules were introduced into the opto-electrostatic micro-vortex, they were stretched around the laser focus. In addition, it was possible to keep it in stretching form for more than 30 sec. Using this method, length of DNA molecules can be measured without fixing to a substrate.

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M3 - Conference contribution

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EP - 1527

BT - Micro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences

PB - Japan Academic Association Inc

T2 - 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006

Y2 - 5 November 2006 through 9 November 2006

ER -

DNA stretching method using a flow induced by laser irradiation under AC electric field

Abstract

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All Science Journal Classification (ASJC) codes

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