DNA separation by cholesterol-bearing pullulan nanogels

Keisuke Kondo; Noritada Kaji; Sayaka Toita; Yukihiro Okamoto; Manabu Tokeshi; Kazunari Akiyoshi; Yoshinobu Baba

doi:10.1063/1.3479997

DNA separation by cholesterol-bearing pullulan nanogels

Keisuke Kondo, Noritada Kaji, Sayaka Toita, Yukihiro Okamoto, Manabu Tokeshi, Kazunari Akiyoshi, Yoshinobu Baba

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

We present an application of a novel DNA separation matrix, cholesterol-bearing pullulan (CHP) nanogels, for microchip electrophoresis. The solution of the CHP showed a unique phase transition around 30 mg/ml and formed gel phase over this critical concentration. This gel phase consists of the weak hydrophobic interactions between the cholesterols could be easily deformed by external forces, and thus, loading process of the CHP nanogels into microchannels became easier. The high concentration of the CHP nanogels provided excellent resolutions especially for small DNA fragments from 100 to 1500 bp. The separation mechanism was discussed based on Ogston and Reptation models which had developed in gels or polymer solutions. The result of a single molecule imaging gave us an insight of the separation mechanism and the nanogel structures as well.

Original language	English
Article number	032210
Journal	Biomicrofluidics
Volume	4
Issue number	3
DOIs	https://doi.org/10.1063/1.3479997
Publication status	Published - 2010
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biomedical Engineering
Materials Science(all)
Condensed Matter Physics
Fluid Flow and Transfer Processes
Colloid and Surface Chemistry

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10.1063/1.3479997

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abstract = "We present an application of a novel DNA separation matrix, cholesterol-bearing pullulan (CHP) nanogels, for microchip electrophoresis. The solution of the CHP showed a unique phase transition around 30 mg/ml and formed gel phase over this critical concentration. This gel phase consists of the weak hydrophobic interactions between the cholesterols could be easily deformed by external forces, and thus, loading process of the CHP nanogels into microchannels became easier. The high concentration of the CHP nanogels provided excellent resolutions especially for small DNA fragments from 100 to 1500 bp. The separation mechanism was discussed based on Ogston and Reptation models which had developed in gels or polymer solutions. The result of a single molecule imaging gave us an insight of the separation mechanism and the nanogel structures as well.",

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T1 - DNA separation by cholesterol-bearing pullulan nanogels

AU - Kondo, Keisuke

AU - Kaji, Noritada

AU - Toita, Sayaka

AU - Okamoto, Yukihiro

AU - Tokeshi, Manabu

AU - Akiyoshi, Kazunari

AU - Baba, Yoshinobu

PY - 2010

Y1 - 2010

N2 - We present an application of a novel DNA separation matrix, cholesterol-bearing pullulan (CHP) nanogels, for microchip electrophoresis. The solution of the CHP showed a unique phase transition around 30 mg/ml and formed gel phase over this critical concentration. This gel phase consists of the weak hydrophobic interactions between the cholesterols could be easily deformed by external forces, and thus, loading process of the CHP nanogels into microchannels became easier. The high concentration of the CHP nanogels provided excellent resolutions especially for small DNA fragments from 100 to 1500 bp. The separation mechanism was discussed based on Ogston and Reptation models which had developed in gels or polymer solutions. The result of a single molecule imaging gave us an insight of the separation mechanism and the nanogel structures as well.

AB - We present an application of a novel DNA separation matrix, cholesterol-bearing pullulan (CHP) nanogels, for microchip electrophoresis. The solution of the CHP showed a unique phase transition around 30 mg/ml and formed gel phase over this critical concentration. This gel phase consists of the weak hydrophobic interactions between the cholesterols could be easily deformed by external forces, and thus, loading process of the CHP nanogels into microchannels became easier. The high concentration of the CHP nanogels provided excellent resolutions especially for small DNA fragments from 100 to 1500 bp. The separation mechanism was discussed based on Ogston and Reptation models which had developed in gels or polymer solutions. The result of a single molecule imaging gave us an insight of the separation mechanism and the nanogel structures as well.

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DNA separation by cholesterol-bearing pullulan nanogels

Abstract

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