TY - GEN
T1 - DNA detection microfluidic device based on negative dielectrophoresis of DNA labeled microbeads
AU - Ding, Zhenhao
AU - Ida, Kenichi
AU - Matsuda, Kenya
AU - Nakano, Michihiko
AU - Suehiro, Junya
N1 - Funding Information:
This work was partly supported by Japan Society for the Promotion of Science KAKENHI Grant Numbers 15K06111 and 26289125.
Publisher Copyright:
© 2017 IEEE.
PY - 2017/12/21
Y1 - 2017/12/21
N2 - In this study, we focus on the alteration of negative dielectrophoresis (n-DEP) caused by small amount of DNA labeling on the microbeads and propose a new design of microfluidic device for more sensitive DNA detection. In previous study, we proposed a new electrical technique for rapid detection of DNA amplified by polymerase chain reaction (PCR). The method is based on dramatic alteration of dielectrophoresis (DEP) characteristics of microbeads caused by DNA labeling. The DNA labeling of microbeads alters their DEP behavior in such a way that they are trapped on a microelectrode under the action of positive DEP (p-DEP), whereas bare microbeads are not, due to the n-DEP. However, this method requires at least 105 copies of DNA labelling on a microbead to alter the DEP behavior of microbeads from negative to positive. As a result, we are able to distinguish bare microbeads and microbeads labeled with DNA at DNA to microbead ratio of 3×103: 1 and 3×104: 1, based on the n-DEP alteration.
AB - In this study, we focus on the alteration of negative dielectrophoresis (n-DEP) caused by small amount of DNA labeling on the microbeads and propose a new design of microfluidic device for more sensitive DNA detection. In previous study, we proposed a new electrical technique for rapid detection of DNA amplified by polymerase chain reaction (PCR). The method is based on dramatic alteration of dielectrophoresis (DEP) characteristics of microbeads caused by DNA labeling. The DNA labeling of microbeads alters their DEP behavior in such a way that they are trapped on a microelectrode under the action of positive DEP (p-DEP), whereas bare microbeads are not, due to the n-DEP. However, this method requires at least 105 copies of DNA labelling on a microbead to alter the DEP behavior of microbeads from negative to positive. As a result, we are able to distinguish bare microbeads and microbeads labeled with DNA at DNA to microbead ratio of 3×103: 1 and 3×104: 1, based on the n-DEP alteration.
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U2 - 10.1109/ICSENS.2017.8234345
DO - 10.1109/ICSENS.2017.8234345
M3 - Conference contribution
AN - SCOPUS:85044255077
T3 - Proceedings of IEEE Sensors
SP - 1
EP - 3
BT - IEEE SENSORS 2017 - Conference Proceedings
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 16th IEEE SENSORS Conference, ICSENS 2017
Y2 - 30 October 2017 through 1 November 2017
ER -