DNA-Damaging Activity and Mutagenicity of Red-Pigment Derived from Ascorbic Acid

Michiko Nonaka; Masata Mitsuiki; Tetsushi Kodama; Hirohisa Omura

doi:10.3358/shokueishi.32.13

DNA-Damaging Activity and Mutagenicity of Red-Pigment Derived from Ascorbic Acid

Michiko Nonaka, Masata Mitsuiki, Tetsushi Kodama, Hirohisa Omura

Food Science and Biotechnology

Research output: Contribution to journal › Article › peer-review

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Abstract

Red-pigment [2,2-nitrilodi-2(2)-deoxy-l-ascorbic acid monoammonium salt (NDA)] was synthesized by the reaction of dehydroascorbic acid with l-phenylalanine. The DNA-damaging activity and the mutagenicity of NDA were assayed by means of DNA-repair and two different gene mutation assays using bacteria. NDA was positive in the spore rec-assay system using Bacillus subtilis without S9 mix, showing that its DNA-damaging index was relatively weak. NDA was positive in both the Ames test and the induced mutation frequency (IMF) assay using Salmonella typhimurium strain TA100 or TA98 with or without S9 mix, revealing that the mutagenic potential was relatively weak. These results suggested that further in vivo tests should be carried out to predict whether NDA might be a carcinogenic or genetic hazard to man.

Original language	English
Pages (from-to)	13
Number of pages	1
Journal	Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi)
Volume	32
Issue number	1
DOIs	https://doi.org/10.3358/shokueishi.32.13
Publication status	Published - 1991

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Food Science
Public Health, Environmental and Occupational Health

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abstract = "Red-pigment [2,2-nitrilodi-2(2)-deoxy-l-ascorbic acid monoammonium salt (NDA)] was synthesized by the reaction of dehydroascorbic acid with l-phenylalanine. The DNA-damaging activity and the mutagenicity of NDA were assayed by means of DNA-repair and two different gene mutation assays using bacteria. NDA was positive in the spore rec-assay system using Bacillus subtilis without S9 mix, showing that its DNA-damaging index was relatively weak. NDA was positive in both the Ames test and the induced mutation frequency (IMF) assay using Salmonella typhimurium strain TA100 or TA98 with or without S9 mix, revealing that the mutagenic potential was relatively weak. These results suggested that further in vivo tests should be carried out to predict whether NDA might be a carcinogenic or genetic hazard to man.",

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AU - Kodama, Tetsushi

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PY - 1991

Y1 - 1991

N2 - Red-pigment [2,2-nitrilodi-2(2)-deoxy-l-ascorbic acid monoammonium salt (NDA)] was synthesized by the reaction of dehydroascorbic acid with l-phenylalanine. The DNA-damaging activity and the mutagenicity of NDA were assayed by means of DNA-repair and two different gene mutation assays using bacteria. NDA was positive in the spore rec-assay system using Bacillus subtilis without S9 mix, showing that its DNA-damaging index was relatively weak. NDA was positive in both the Ames test and the induced mutation frequency (IMF) assay using Salmonella typhimurium strain TA100 or TA98 with or without S9 mix, revealing that the mutagenic potential was relatively weak. These results suggested that further in vivo tests should be carried out to predict whether NDA might be a carcinogenic or genetic hazard to man.

AB - Red-pigment [2,2-nitrilodi-2(2)-deoxy-l-ascorbic acid monoammonium salt (NDA)] was synthesized by the reaction of dehydroascorbic acid with l-phenylalanine. The DNA-damaging activity and the mutagenicity of NDA were assayed by means of DNA-repair and two different gene mutation assays using bacteria. NDA was positive in the spore rec-assay system using Bacillus subtilis without S9 mix, showing that its DNA-damaging index was relatively weak. NDA was positive in both the Ames test and the induced mutation frequency (IMF) assay using Salmonella typhimurium strain TA100 or TA98 with or without S9 mix, revealing that the mutagenic potential was relatively weak. These results suggested that further in vivo tests should be carried out to predict whether NDA might be a carcinogenic or genetic hazard to man.

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DNA-Damaging Activity and Mutagenicity of Red-Pigment Derived from Ascorbic Acid

Abstract

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