Distribution of tetracaine and its metabolite in rabbits after high versus normal spinal anesthesia

Yukiko Hino, Hidefumi Inoue, Keiko Kudo, Naoki Nishida, Noriaki Ikeda

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)


High spinal anesthesia is one cause of sudden death associated with the spinal anesthesia. We did animal experiments to verify high spinal anesthesia by analyzing tetracaine and its metabolite, p-butylaminobenzoic acid in tissue samples. Tetracaine (0.25% in 10% glucose solution) 0.21-0.28mg/kg was administered to two groups of rabbits to induce high and normal spinal anesthesia. Tetracaine and the metabolite in rabbit tissues were analyzed by gas chromatography-mass spectrometry, as a free base for tetracaine and as tert-butyldimethylsilyl derivative for the metabolite. In the group given high spinal anesthesia, levels of the metabolite in the brain stem were higher than in the cerebrum, cerebellum and whole blood. On the other hand, in the group given normal spinal anesthesia, the opposite results were obtained. Therefore, high spinal anesthesia induced by tetracaine can be diagnosed by comparing the concentrations of metabolite in whole blood, cerebrum, cerebellum and brain stem.

Original languageEnglish
Pages (from-to)130-136
Number of pages7
JournalForensic Science International
Issue number2-3
Publication statusPublished - Dec 27 2001

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine


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