TY - JOUR
T1 - Distinct roles of the Src family kinases, SRC-1 and KIN-22, that are negatively regulated by CSK-1 in C. elegans
AU - Hirose, Takashi
AU - Koga, Makoto
AU - Ohshima, Yasumi
AU - Okada, Masato
N1 - Funding Information:
We thank J.A. Cooper for discussions and comments on the manuscript, H. Sawa for germline transformation, Yuji Kohara for EST clones, A. Coulson and J. Sulstone for physical mapping, cosmid and YAC clones, and A. Fire for the vectors. We also thank members of our laboratory for providing materials and valuable discussion. Some of the strains used in this work were provided by the Caenorhabditis Genetics Center, which is funded by the National Institutes of Health Center for Research Resources. This work was supported in part by grants-in-aid from the Ministry of Education, Science, Technology, Sports and Culture of Japan and by grants from the Nagase Science and Technology Foundation, The Mitsubishi Foundation, Japan Research Foundation for Clinical Pharmacology, and The Program of Fundamental Studies in Health Science of the Organization for Pharmaceutical Safety and Research.
PY - 2003/1/16
Y1 - 2003/1/16
N2 - To elucidate the primitive roles of the Src family kinases (SFKs), here we characterized Caenorhabditis elegans orthologues of SFKs (src-1 and kin-22) and their regulator kinase Csk (csk-1). SRC-1 and KIN-22 possess the C-terminal regulatory tyrosines characteristic of SFKs, and their activities are negatively regulated by CSK-1 in a yeast expression system. The src-1 and csk-1 genes are co-expressed in some head neurons, the anchor cell and the tail region, while kin-22 and csk-1 genes are co-expressed in pharyngeal muscles and tail region. Expression of KIN-22 induced morphological defects in the pharynx, whereas expression of SRC-1 did not show any overt phenotype in adult. RNA interference of src-1, but not that of kin-22, caused a developmental arrest in early development. These results suggest that SRC-1 and KIN-22 play distinct roles under the control of CSK-1.
AB - To elucidate the primitive roles of the Src family kinases (SFKs), here we characterized Caenorhabditis elegans orthologues of SFKs (src-1 and kin-22) and their regulator kinase Csk (csk-1). SRC-1 and KIN-22 possess the C-terminal regulatory tyrosines characteristic of SFKs, and their activities are negatively regulated by CSK-1 in a yeast expression system. The src-1 and csk-1 genes are co-expressed in some head neurons, the anchor cell and the tail region, while kin-22 and csk-1 genes are co-expressed in pharyngeal muscles and tail region. Expression of KIN-22 induced morphological defects in the pharynx, whereas expression of SRC-1 did not show any overt phenotype in adult. RNA interference of src-1, but not that of kin-22, caused a developmental arrest in early development. These results suggest that SRC-1 and KIN-22 play distinct roles under the control of CSK-1.
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U2 - 10.1016/S0014-5793(02)03819-X
DO - 10.1016/S0014-5793(02)03819-X
M3 - Article
C2 - 12527374
AN - SCOPUS:12244271449
SN - 0014-5793
VL - 534
SP - 133
EP - 138
JO - FEBS Letters
JF - FEBS Letters
IS - 1-3
ER -