TY - JOUR
T1 - Disruption of the hslU gene, which encodes an ATPase subunit of the eukaryotic 26S proteasome homolog in Escherichia coli, suppresses the temperature-sensitive dnaA46 mutation
AU - Katayama, Tsutomu
AU - Kubota, Toshio
AU - Takata, Makoto
AU - Akimitsu, Nobuyoshi
AU - Sekimizu, Kazuhisa
N1 - Funding Information:
Bacterial strains, phages, and plasmids. All strains were derivatives of Escherichia coli K12. Genotype of KH5402-1 is: thyA thr trpE9829(Am) ilv tyrA(Am) metE deo supF6(Ts) (19). KA456, KA459, and KA460 are derivatives of KH5402-1 carrying clpP1::CmR (20), hslU1::mini-tet, and lon::Tn10, respectively. The lon::Tn10 mutation is 1Recipient of a predoctoral fellowship from the Japanese Society for Promotion of Science. 2To whom correspondence should be addressed. Fax: /81-92-641-6648.
Funding Information:
We are grateful to Dr. S. Hiraga for suggestions and assistance with experiments using a flowcytometer and Dr. A. Nishimura for strains. This work was supported in part by a Grant-in-Aid for Scientific Research on the Priority Areas from the Ministry of Education, Science, Sports, and Culture of Japan.
PY - 1996/12/4
Y1 - 1996/12/4
N2 - The Escherichia coil hslVU genes encode subunits of an ATP-dependent protease, a homolog of the eukaryotic 265 proteasome. We found that the hslU gene is required for the temperature (40°C)-sensitive phenotype of the dnaA46 mutant, while other soluble ATP-dependent proteases, La (Lon) and Ti (Clp), were unrelated to this dnaA46 phenotype. Disruption of the hslU gene inhibited cell growth at high temperatures. These observations suggest a specific in vivo role for HslVU protease in denatured proteins. As the absence of HslU produces minicells in M9 medium, the protease may be involved in the cell cycle regulation.
AB - The Escherichia coil hslVU genes encode subunits of an ATP-dependent protease, a homolog of the eukaryotic 265 proteasome. We found that the hslU gene is required for the temperature (40°C)-sensitive phenotype of the dnaA46 mutant, while other soluble ATP-dependent proteases, La (Lon) and Ti (Clp), were unrelated to this dnaA46 phenotype. Disruption of the hslU gene inhibited cell growth at high temperatures. These observations suggest a specific in vivo role for HslVU protease in denatured proteins. As the absence of HslU produces minicells in M9 medium, the protease may be involved in the cell cycle regulation.
UR - http://www.scopus.com/inward/record.url?scp=0030569512&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030569512&partnerID=8YFLogxK
U2 - 10.1006/bbrc.1996.1783
DO - 10.1006/bbrc.1996.1783
M3 - Article
C2 - 8954109
AN - SCOPUS:0030569512
SN - 0006-291X
VL - 229
SP - 219
EP - 224
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -