TY - JOUR
T1 - Discrimination of the oral microbiota associated with high hydrogen sulfide and methyl mercaptan production
AU - Takeshita, Toru
AU - Suzuki, Nao
AU - Nakano, Yoshio
AU - Yasui, Masaki
AU - Yoneda, Masahiro
AU - Shimazaki, Yoshihiro
AU - Hirofuji, Takao
AU - Yamashita, Yoshihisa
N1 - Funding Information:
This study was supported in part by Grants-in Aid for Young Scientist 23792517 (T. T.) and 23792532 (N. S.), by Grants-in Aid for Scientific Research 21592652 (Y. N.), 20592249 (M. Y.), 22406034 (Y. S.), 23593078 (T. H.) and 23659986 (Y. Y.) and by Grants-in Aid for Scientific Research (Strategic Research Promotion) from the Ministry of Education, Culture, Sports, Science and Technology of Japan as well as by the Sato Fund from the Nihon University School of Dentistry (Y. N.) and by the Uehara Memorial Foundation (Y. Y.).
PY - 2012
Y1 - 2012
N2 - Both hydrogen sulfide (H 2S) and methyl mercaptan (CH 3SH) are frequently detected in large amounts in malodorous mouth air. We investigated the bacterial composition of saliva of 30 subjects with severe oral malodor exhibiting extreme CH 3SH/H 2S ratios (high H 2S but low CH 3SH concentrations, n = 14; high CH 3SH but low H 2S concentrations, n = 16) and 13 subjects without malodor, using barcoded pyrosequencing analysis of the 16S rRNA gene. Phylogenetic community analysis with the UniFrac distance metric revealed a distinct bacterial community structure in each malodor group. The H 2S group showed higher proportions of the genera Neisseria, Fusobacterium, Porphyromonas and SR1 than the other two groups, whereas the CH 3SH group had higher proportions of the genera Prevotella, Veillonella, Atopobium, Megasphaera, and Selenomonas. Our results suggested that distinct bacterial populations in the oral microbiota are involved in production of high levels of H 2S and CH 3SH in the oral cavity.
AB - Both hydrogen sulfide (H 2S) and methyl mercaptan (CH 3SH) are frequently detected in large amounts in malodorous mouth air. We investigated the bacterial composition of saliva of 30 subjects with severe oral malodor exhibiting extreme CH 3SH/H 2S ratios (high H 2S but low CH 3SH concentrations, n = 14; high CH 3SH but low H 2S concentrations, n = 16) and 13 subjects without malodor, using barcoded pyrosequencing analysis of the 16S rRNA gene. Phylogenetic community analysis with the UniFrac distance metric revealed a distinct bacterial community structure in each malodor group. The H 2S group showed higher proportions of the genera Neisseria, Fusobacterium, Porphyromonas and SR1 than the other two groups, whereas the CH 3SH group had higher proportions of the genera Prevotella, Veillonella, Atopobium, Megasphaera, and Selenomonas. Our results suggested that distinct bacterial populations in the oral microbiota are involved in production of high levels of H 2S and CH 3SH in the oral cavity.
UR - http://www.scopus.com/inward/record.url?scp=84859743410&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84859743410&partnerID=8YFLogxK
U2 - 10.1038/srep00215
DO - 10.1038/srep00215
M3 - Article
C2 - 22355729
AN - SCOPUS:84859743410
SN - 2045-2322
VL - 2
JO - Scientific reports
JF - Scientific reports
M1 - 215
ER -