Discrimination of sugi (Cryptomeria japonica D. Don) plus-tree clones using SSR marker analysis

T. Hirao; A. Watanabe; Y. Goto-Fukuda; T. Kondo; K. Takata

doi:10.4005/jjfs.88.202

Discrimination of sugi (Cryptomeria japonica D. Don) plus-tree clones using SSR marker analysis

T. Hirao, A. Watanabe, Y. Goto-Fukuda, T. Kondo, K. Takata

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Eight hundred and thirty-five sugi (Cryptomerica japonica D. Don) plus-tree clones from the Kanto Breeding Region were genotyped using three highly polymorphic SSR markers. With a mean observed heterozygosity of 0.831 and discrimination power at a locus varying from 0.98 to 0.99, the SSR markers were more effective for discrimination than RAPD (Random Amplified Polymorphic DNA) markers. Although five other markers were added to discriminate the remaining 189 sugi plus-tree that indicated the same genotype using three markers, only 6 plus-tree clones possessed the original genotype. One of 8 SSR markers, Cjgssr149, seemed to be multi-locus, and the SSR motifs of two clones were sequenced to verified whether it was multi-locus or not. As a result, it revealed that a duplicated SSR region was amplified.

Original language	English
Pages (from-to)	202-205
Number of pages	4
Journal	Nihon Ringakkai Shi/Journal of the Japanese Forestry Society
Volume	88
Issue number	3
DOIs	https://doi.org/10.4005/jjfs.88.202
Publication status	Published - 2006
Externally published	Yes

All Science Journal Classification (ASJC) codes

Forestry

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10.4005/jjfs.88.202

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title = "Discrimination of sugi (Cryptomeria japonica D. Don) plus-tree clones using SSR marker analysis",

abstract = "Eight hundred and thirty-five sugi (Cryptomerica japonica D. Don) plus-tree clones from the Kanto Breeding Region were genotyped using three highly polymorphic SSR markers. With a mean observed heterozygosity of 0.831 and discrimination power at a locus varying from 0.98 to 0.99, the SSR markers were more effective for discrimination than RAPD (Random Amplified Polymorphic DNA) markers. Although five other markers were added to discriminate the remaining 189 sugi plus-tree that indicated the same genotype using three markers, only 6 plus-tree clones possessed the original genotype. One of 8 SSR markers, Cjgssr149, seemed to be multi-locus, and the SSR motifs of two clones were sequenced to verified whether it was multi-locus or not. As a result, it revealed that a duplicated SSR region was amplified.",

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AU - Hirao, T.

AU - Watanabe, A.

AU - Goto-Fukuda, Y.

AU - Kondo, T.

AU - Takata, K.

PY - 2006

Y1 - 2006

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Discrimination of sugi (Cryptomeria japonica D. Don) plus-tree clones using SSR marker analysis

Abstract

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