Discovery of highly reactive peptide tag by ELISA-type screening for specific cysteine conjugation

Nobutaka Kurashige; Hirokazu Fuchida; Shigekazu Tabata; Shohei Uchinomiya; Akio Ojida

doi:10.1016/j.bmcl.2017.05.069

Discovery of highly reactive peptide tag by ELISA-type screening for specific cysteine conjugation

Nobutaka Kurashige, Hirokazu Fuchida, Shigekazu Tabata, Shohei Uchinomiya, Akio Ojida

Research output: Contribution to journal › Article › peer-review

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Abstract

We report the discovery of a highly reactive peptide tag for the specific cysteine conjugation of proteins. Screening of cysteine-containing peptides using ELISA-type screening yielded a 19-amino acid tag (DCPPPDDAADDAADDAADD), named DCP3 tag, which enabled the rapid and selective labeling of the tag-fused protein with a synthetic zinc complex on the surface of living cells.

Original language	English
Pages (from-to)	3486-3489
Number of pages	4
Journal	Bioorganic and Medicinal Chemistry Letters
Volume	27
Issue number	15
DOIs	https://doi.org/10.1016/j.bmcl.2017.05.069
Publication status	Published - 2017

All Science Journal Classification (ASJC) codes

Biochemistry
Molecular Medicine
Molecular Biology
Pharmaceutical Science
Drug Discovery
Clinical Biochemistry
Organic Chemistry

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10.1016/j.bmcl.2017.05.069

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abstract = "We report the discovery of a highly reactive peptide tag for the specific cysteine conjugation of proteins. Screening of cysteine-containing peptides using ELISA-type screening yielded a 19-amino acid tag (DCPPPDDAADDAADDAADD), named DCP3 tag, which enabled the rapid and selective labeling of the tag-fused protein with a synthetic zinc complex on the surface of living cells.",

author = "Nobutaka Kurashige and Hirokazu Fuchida and Shigekazu Tabata and Shohei Uchinomiya and Akio Ojida",

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AU - Tabata, Shigekazu

AU - Uchinomiya, Shohei

AU - Ojida, Akio

PY - 2017

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AB - We report the discovery of a highly reactive peptide tag for the specific cysteine conjugation of proteins. Screening of cysteine-containing peptides using ELISA-type screening yielded a 19-amino acid tag (DCPPPDDAADDAADDAADD), named DCP3 tag, which enabled the rapid and selective labeling of the tag-fused protein with a synthetic zinc complex on the surface of living cells.

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ER -

Discovery of highly reactive peptide tag by ELISA-type screening for specific cysteine conjugation

Abstract

All Science Journal Classification (ASJC) codes

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