Disappearance of the lymphoid system in Bcl-2 homozygous mutant chimeric mice

Kei Ichi Nakayama; Keiko Nakayama; Izumi Negishi; Keisuke Kuida; Yoichi Shinkai; Marjorie C. Louie; Larry E. Fields; Phillip J. Lucas; Valerie Stewart; Frederick W. Alt; Dennis Y. Loh

doi:10.1126/science.8372353

Disappearance of the lymphoid system in Bcl-2 homozygous mutant chimeric mice

Kei Ichi Nakayama, Keiko Nakayama, Izumi Negishi, Keisuke Kuida, Yoichi Shinkai, Marjorie C. Louie, Larry E. Fields, Phillip J. Lucas, Valerie Stewart, Frederick W. Alt, Dennis Y. Loh

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379 Citations (Scopus)

Abstract

The bcl-2 proto-oncogene can prevent the death of many cell types. Mice were generated that were chimeric for the homozygous inactivation of bcl-2. Lymphocytes without Bcl-2 differentiated into phenotypically mature cells. However, in vitro, the mature T cells that lacked Bcl-2 had shorter life-spans and increased sensitivity to glucocorticoids and γ-irradiation. In contrast, stimulation of CD3 inhibited the death of these cells. T and B cells with no Bcl-2 disappeared from the bone marrow, thymus, and periphery by 4 weeks of age. Thus, Bcl-2 was dispensable for lymphocyte maturation, but was required for a stable immune system after birth.

Original language	English
Pages (from-to)	1584-1588
Number of pages	5
Journal	Science
Volume	261
Issue number	5128
DOIs	https://doi.org/10.1126/science.8372353
Publication status	Published - 1993
Externally published	Yes

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title = "Disappearance of the lymphoid system in Bcl-2 homozygous mutant chimeric mice",

abstract = "The bcl-2 proto-oncogene can prevent the death of many cell types. Mice were generated that were chimeric for the homozygous inactivation of bcl-2. Lymphocytes without Bcl-2 differentiated into phenotypically mature cells. However, in vitro, the mature T cells that lacked Bcl-2 had shorter life-spans and increased sensitivity to glucocorticoids and γ-irradiation. In contrast, stimulation of CD3 inhibited the death of these cells. T and B cells with no Bcl-2 disappeared from the bone marrow, thymus, and periphery by 4 weeks of age. Thus, Bcl-2 was dispensable for lymphocyte maturation, but was required for a stable immune system after birth.",

author = "Nakayama, {Kei Ichi} and Keiko Nakayama and Izumi Negishi and Keisuke Kuida and Yoichi Shinkai and Louie, {Marjorie C.} and Fields, {Larry E.} and Lucas, {Phillip J.} and Valerie Stewart and Alt, {Frederick W.} and Loh, {Dennis Y.}",

year = "1993",

doi = "10.1126/science.8372353",

language = "English",

volume = "261",

pages = "1584--1588",

journal = "Science",

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T1 - Disappearance of the lymphoid system in Bcl-2 homozygous mutant chimeric mice

AU - Nakayama, Kei Ichi

AU - Nakayama, Keiko

AU - Negishi, Izumi

AU - Kuida, Keisuke

AU - Shinkai, Yoichi

AU - Louie, Marjorie C.

AU - Fields, Larry E.

AU - Lucas, Phillip J.

AU - Stewart, Valerie

AU - Alt, Frederick W.

AU - Loh, Dennis Y.

PY - 1993

Y1 - 1993

N2 - The bcl-2 proto-oncogene can prevent the death of many cell types. Mice were generated that were chimeric for the homozygous inactivation of bcl-2. Lymphocytes without Bcl-2 differentiated into phenotypically mature cells. However, in vitro, the mature T cells that lacked Bcl-2 had shorter life-spans and increased sensitivity to glucocorticoids and γ-irradiation. In contrast, stimulation of CD3 inhibited the death of these cells. T and B cells with no Bcl-2 disappeared from the bone marrow, thymus, and periphery by 4 weeks of age. Thus, Bcl-2 was dispensable for lymphocyte maturation, but was required for a stable immune system after birth.

AB - The bcl-2 proto-oncogene can prevent the death of many cell types. Mice were generated that were chimeric for the homozygous inactivation of bcl-2. Lymphocytes without Bcl-2 differentiated into phenotypically mature cells. However, in vitro, the mature T cells that lacked Bcl-2 had shorter life-spans and increased sensitivity to glucocorticoids and γ-irradiation. In contrast, stimulation of CD3 inhibited the death of these cells. T and B cells with no Bcl-2 disappeared from the bone marrow, thymus, and periphery by 4 weeks of age. Thus, Bcl-2 was dispensable for lymphocyte maturation, but was required for a stable immune system after birth.

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Disappearance of the lymphoid system in Bcl-2 homozygous mutant chimeric mice

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