Directed aggregation and fusion of lipid vesicles induced by DNA-surfactants

Tatsuo Maruyama, Hiroshi Yamamura, Mai Hiraki, Yoshinori Kemori, Harumi Takata, Masahiro Goto

    Research output: Contribution to journalArticlepeer-review

    25 Citations (Scopus)


    We investigated DNA-directed aggregation of vesicles using DNA-surfactants. Following tethering of single-stranded DNA oligonucleotides to vesicles using DNA-surfactant, the tethered vesicles were assembled with other vesicles bearing complementary strands. The vesicle aggregation was strongly affected by the salt concentration and by temperature according to the characteristics of DNA hybridization. Restriction enzyme, which can hydrolyze the double-stranded DNA used in the present study, dissociated the vesicle aggregates. Exploration using fluorescently labeled vesicles suggested that the DNA-directed vesicle aggregation took place in a sequence-specific manner through DNA-duplex formation. Interestingly, the DNA-directed aggregation using short DNA-surfactant induced the fusion of vesicles to produce giant vesicles, resulting in an enzymatic reaction in the giant vesicle.

    Original languageEnglish
    Pages (from-to)119-124
    Number of pages6
    JournalColloids and Surfaces B: Biointerfaces
    Issue number1
    Publication statusPublished - Oct 1 2008

    All Science Journal Classification (ASJC) codes

    • Biotechnology
    • Surfaces and Interfaces
    • Physical and Theoretical Chemistry
    • Colloid and Surface Chemistry


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