Direct reprogramming of non-limb fibroblasts to cells with properties of limb progenitors

Yuji Atsuta; Chang Hee Lee; Alan R. Rodrigues; Charlotte Colle; Reiko R. Tomizawa; Ernesto G. Lujan; Patrick Tschopp; Laura Galan; Meng Zhu; Joshua M. Gorham; Jean Pierre Vannier; Christine E. Seidman; Jonathan G. Seidman; Marian A. Ros; Olivier Pourquié; Clifford J. Tabin

doi:10.1016/j.devcel.2023.12.010

Direct reprogramming of non-limb fibroblasts to cells with properties of limb progenitors

Yuji Atsuta, Chang Hee Lee, Alan R. Rodrigues, Charlotte Colle, Reiko R. Tomizawa, Ernesto G. Lujan, Patrick Tschopp, Laura Galan, Meng Zhu, Joshua M. Gorham, Jean Pierre Vannier, Christine E. Seidman, Jonathan G. Seidman, Marian A. Ros, Olivier Pourquié, Clifford J. Tabin

Informational Biology

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

The early limb bud consists of mesenchymal limb progenitors derived from the lateral plate mesoderm (LPM). The LPM also gives rise to the mesodermal components of the flank and neck. However, the cells at these other levels cannot produce the variety of cell types found in the limb. Taking advantage of a direct reprogramming approach, we find a set of factors (Prdm16, Zbtb16, and Lin28a) normally expressed in the early limb bud and capable of imparting limb progenitor-like properties to mouse non-limb fibroblasts. The reprogrammed cells show similar gene expression profiles and can differentiate into similar cell types as endogenous limb progenitors. The further addition of Lin41 potentiates the proliferation of the reprogrammed cells. These results suggest that these same four factors may play pivotal roles in the specification of endogenous limb progenitors.

Original language	English
Pages (from-to)	415-430.e8
Journal	Developmental Cell
Volume	59
Issue number	3
DOIs	https://doi.org/10.1016/j.devcel.2023.12.010
Publication status	Published - Feb 5 2024

All Science Journal Classification (ASJC) codes

Molecular Biology
General Biochemistry,Genetics and Molecular Biology
Developmental Biology
Cell Biology

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10.1016/j.devcel.2023.12.010

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Atsuta, Y., Lee, C. H., Rodrigues, A. R., Colle, C., Tomizawa, R. R., Lujan, E. G., Tschopp, P., Galan, L., Zhu, M., Gorham, J. M., Vannier, J. P., Seidman, C. E., Seidman, J. G., Ros, M. A., Pourquié, O., & Tabin, C. J. (2024). Direct reprogramming of non-limb fibroblasts to cells with properties of limb progenitors. Developmental Cell, 59(3), 415-430.e8. https://doi.org/10.1016/j.devcel.2023.12.010

Atsuta, Y, Lee, CH, Rodrigues, AR, Colle, C, Tomizawa, RR, Lujan, EG, Tschopp, P, Galan, L, Zhu, M, Gorham, JM, Vannier, JP, Seidman, CE, Seidman, JG, Ros, MA, Pourquié, O & Tabin, CJ 2024, 'Direct reprogramming of non-limb fibroblasts to cells with properties of limb progenitors', Developmental Cell, vol. 59, no. 3, pp. 415-430.e8. https://doi.org/10.1016/j.devcel.2023.12.010

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title = "Direct reprogramming of non-limb fibroblasts to cells with properties of limb progenitors",

abstract = "The early limb bud consists of mesenchymal limb progenitors derived from the lateral plate mesoderm (LPM). The LPM also gives rise to the mesodermal components of the flank and neck. However, the cells at these other levels cannot produce the variety of cell types found in the limb. Taking advantage of a direct reprogramming approach, we find a set of factors (Prdm16, Zbtb16, and Lin28a) normally expressed in the early limb bud and capable of imparting limb progenitor-like properties to mouse non-limb fibroblasts. The reprogrammed cells show similar gene expression profiles and can differentiate into similar cell types as endogenous limb progenitors. The further addition of Lin41 potentiates the proliferation of the reprogrammed cells. These results suggest that these same four factors may play pivotal roles in the specification of endogenous limb progenitors.",

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author = "Yuji Atsuta and Lee, {Chang Hee} and Rodrigues, {Alan R.} and Charlotte Colle and Tomizawa, {Reiko R.} and Lujan, {Ernesto G.} and Patrick Tschopp and Laura Galan and Meng Zhu and Gorham, {Joshua M.} and Vannier, {Jean Pierre} and Seidman, {Christine E.} and Seidman, {Jonathan G.} and Ros, {Marian A.} and Olivier Pourqui{\'e} and Tabin, {Clifford J.}",

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AU - Tomizawa, Reiko R.

AU - Lujan, Ernesto G.

AU - Tschopp, Patrick

AU - Galan, Laura

AU - Zhu, Meng

AU - Gorham, Joshua M.

AU - Vannier, Jean Pierre

AU - Seidman, Christine E.

AU - Seidman, Jonathan G.

AU - Ros, Marian A.

AU - Pourquié, Olivier

AU - Tabin, Clifford J.

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N2 - The early limb bud consists of mesenchymal limb progenitors derived from the lateral plate mesoderm (LPM). The LPM also gives rise to the mesodermal components of the flank and neck. However, the cells at these other levels cannot produce the variety of cell types found in the limb. Taking advantage of a direct reprogramming approach, we find a set of factors (Prdm16, Zbtb16, and Lin28a) normally expressed in the early limb bud and capable of imparting limb progenitor-like properties to mouse non-limb fibroblasts. The reprogrammed cells show similar gene expression profiles and can differentiate into similar cell types as endogenous limb progenitors. The further addition of Lin41 potentiates the proliferation of the reprogrammed cells. These results suggest that these same four factors may play pivotal roles in the specification of endogenous limb progenitors.

AB - The early limb bud consists of mesenchymal limb progenitors derived from the lateral plate mesoderm (LPM). The LPM also gives rise to the mesodermal components of the flank and neck. However, the cells at these other levels cannot produce the variety of cell types found in the limb. Taking advantage of a direct reprogramming approach, we find a set of factors (Prdm16, Zbtb16, and Lin28a) normally expressed in the early limb bud and capable of imparting limb progenitor-like properties to mouse non-limb fibroblasts. The reprogrammed cells show similar gene expression profiles and can differentiate into similar cell types as endogenous limb progenitors. The further addition of Lin41 potentiates the proliferation of the reprogrammed cells. These results suggest that these same four factors may play pivotal roles in the specification of endogenous limb progenitors.

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Direct reprogramming of non-limb fibroblasts to cells with properties of limb progenitors

Abstract

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