Direct monitoring of EcoRII restriction enzyme reactions on a 27 MHz quartz-crystal microbalance.

Shuntaro Takahashi; Hisao Matsuno; Hiroyuki Furusawa; Yoshio Okahata

doi:10.1093/nass/2.1.71

Direct monitoring of EcoRII restriction enzyme reactions on a 27 MHz quartz-crystal microbalance.

Shuntaro Takahashi, Hisao Matsuno, Hiroyuki Furusawa, Yoshio Okahata

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

EcoRII is a type IIe restriction endonuclease characterized by a highly cooperative reaction mechanism that depends on simultaneous binding of the dimeric enzyme molecule to two copies of its recognition site. Gaining insights about reaction property of EcoRII binding to its recognition site and effect of divalent metal ion on DNA cleavage process, a DNA-immobilized quartz crystal microbalance (QCM), which enables real-time monitoring both the binding of enzyme and the cleavage reaction on DNA strands as mass changes, was utilized.

Original language	English
Pages (from-to)	71-72
Number of pages	2
Journal	Nucleic acids research. Supplement (2001)
Issue number	2
DOIs	https://doi.org/10.1093/nass/2.1.71
Publication status	Published - 2002
Externally published	Yes

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abstract = "EcoRII is a type IIe restriction endonuclease characterized by a highly cooperative reaction mechanism that depends on simultaneous binding of the dimeric enzyme molecule to two copies of its recognition site. Gaining insights about reaction property of EcoRII binding to its recognition site and effect of divalent metal ion on DNA cleavage process, a DNA-immobilized quartz crystal microbalance (QCM), which enables real-time monitoring both the binding of enzyme and the cleavage reaction on DNA strands as mass changes, was utilized.",

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AU - Matsuno, Hisao

AU - Furusawa, Hiroyuki

AU - Okahata, Yoshio

PY - 2002

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AB - EcoRII is a type IIe restriction endonuclease characterized by a highly cooperative reaction mechanism that depends on simultaneous binding of the dimeric enzyme molecule to two copies of its recognition site. Gaining insights about reaction property of EcoRII binding to its recognition site and effect of divalent metal ion on DNA cleavage process, a DNA-immobilized quartz crystal microbalance (QCM), which enables real-time monitoring both the binding of enzyme and the cleavage reaction on DNA strands as mass changes, was utilized.

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JO - Nucleic acids research. Supplement (2001)

JF - Nucleic acids research. Supplement (2001)

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ER -

Direct monitoring of EcoRII restriction enzyme reactions on a 27 MHz quartz-crystal microbalance.

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