Direct analysis of thermophilic bacteria in sewage sludge compost

Kota Shiotsuka, Shinjiro Kanazawa, Kenji Sakai

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3 Citations (Scopus)


To analyze metabolically active thermophilic bacteria, a high-temperature direct viable count (HT-DVC) method was developed and applied to sewage sludge compost made by a hyperthermal composting method. When the HT-DVC method was conducted at 60°C and 80°C, maximum numbers of 23.3×108 and 2.62×108 cells/(g of dry sample) of elongated cells (length > 4μm), respectively, were detected. These results indicate that the HT-DVC method can be used to enumerate even metabolically active extreme thermopniles. Strain TH, a Gram-negative, spore-forming, and extremely thermophilic bacterium, which showed growth at 55-78°C, was isolated from the sewage sludge compost. Strain TH is closely related to Caldaterra satsumae YM081. The HT-DVC method could detect strain TH inoculated into sewage sludge compost with autoclaving, but could not selectively detect the strain inoculated into the compost without autoclaving.

Original languageEnglish
Pages (from-to)471-477
Number of pages7
JournalJournal of the Faculty of Agriculture, Kyushu University
Issue number2
Publication statusPublished - Oct 2008

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science


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