Differentiation of phytopathogenic Pseudomonas and Xanthomonas pathovars and strains by PCR analysis for DNA topoisomerase genes

Abu Ashraf Khan, Naruto Furuya, Masaru Matsumoto, Nobuaki Matsuyama

Research output: Contribution to journalArticlepeer-review

Abstract

Differentiation of pathovars of Pseudomonas syringae and Xanthomonas campestris was conducted by analysis with polymerase chain reaction (PCR) of topoismerase genes. Differences among the pathovars were observed on the migration patterns of the PCR products on agarose gel. Banding patterns of respective strains were pathovar specific with some exceptions. The technique is rapid, simple and reproductive to identify and classify phytopathogenic Pseudomonas syringae and Xanthomonas campestris at pathovar level, and it may be a useful diagnostic tool for these important plant pathogens.

Original languageEnglish
Pages (from-to)1-6
Number of pages6
JournalJournal of the Faculty of Agriculture, Kyushu University
Volume45
Issue number1
DOIs
Publication statusPublished - Nov 2000

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science

Fingerprint

Dive into the research topics of 'Differentiation of phytopathogenic Pseudomonas and Xanthomonas pathovars and strains by PCR analysis for DNA topoisomerase genes'. Together they form a unique fingerprint.

Cite this