Differentiation of induced pluripotent stem cells into dental mesenchymal cells

Keishi Otsu, Ryota Kishigami, Ai Oikawa-Sasaki, Satoshi Fukumoto, Aya Yamada, Naoki Fujiwara, Kiyoto Ishizeki, Hidemitsu Harada

Research output: Contribution to journalArticlepeer-review

104 Citations (Scopus)


Similar to embryonic stem cells, induced pluripotent stem (iPS) cells can differentiate into various cell types upon appropriate induction, and thus, may be valuable cell sources for regenerative medicine. However, iPS cells have not been reported to differentiate into odontogenic cells for tooth regeneration. Here we demonstrated that neural crest-like cells (NCLC) derived from mouse iPS cells have the potential to differentiate into odontogenic mesenchymal cells. We developed an efficient culture protocol to induce the differentiation of mouse iPS cells into NCLC. We confirmed that the cells exhibited neural crest (NC) cell markers as evidenced by immunocytochemistry, flow cytometry, and real-time reverse transcription-polymerase chain reaction. Further, in recombination cultures of NCLC and mouse dental epithelium, NCLC exhibited a gene expression pattern involving dental mesenchymal cells. Some NCLC also expressed dentin sialoprotein. Conditioned medium of mouse dental epithelium cultures further enhanced the differentiation of NCLC into odontoblasts. These results suggest that iPS cells are useful cell sources for tooth regeneration and tooth development studies.

Original languageEnglish
Pages (from-to)1156-1164
Number of pages9
JournalStem cells and development
Issue number7
Publication statusPublished - May 1 2012
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Hematology
  • Developmental Biology
  • Cell Biology


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