TY - JOUR
T1 - Differentiation of circulating endothelial progenitor cells to a cardiomyogenic phenotype depends on E-cadherin
AU - Koyanagi, Masamichi
AU - Urbich, Carmen
AU - Chavakis, Emmanouil
AU - Hoffmann, Jörg
AU - Rupp, Stefan
AU - Badorff, Cornel
AU - Zeiher, Andreas M.
AU - Starzinski-Powitz, Anna
AU - Haendeler, Judith
AU - Dimmeler, Stefanie
N1 - Funding Information:
We thank Iris Stügelmaier and Christine Goy for expert technical assistance. This study was supported by the DFG to S.D. (Di 600/6-1), the Japan Heart Foundation/Bayer Yakuhin Research Grant Abroad to M.K.
PY - 2005/11/7
Y1 - 2005/11/7
N2 - Progenitor cells may contribute to cardiac regeneration. Here, we investigated the role of cadherins and integrins for differentiation of human adult circulating endothelial progenitor cells (EPCs) into cardiomyocytes (CM) in a co-culture system. N- and E-cadherin were expressed in EPCs and were localized at the interface between EPCs and CM. Incubation of a blocking antibody against E-cadherin reduced the expression of CM marker protein in EPCs. Blocking antibodies against N- or P-cadherin or the β1- and β2-integrins were not effective. These data suggested that cell-to-cell communication mediated by E-cadherin contributes to the acquirement of a cardiomyogenic phenotype of human endothelial progenitor cells.
AB - Progenitor cells may contribute to cardiac regeneration. Here, we investigated the role of cadherins and integrins for differentiation of human adult circulating endothelial progenitor cells (EPCs) into cardiomyocytes (CM) in a co-culture system. N- and E-cadherin were expressed in EPCs and were localized at the interface between EPCs and CM. Incubation of a blocking antibody against E-cadherin reduced the expression of CM marker protein in EPCs. Blocking antibodies against N- or P-cadherin or the β1- and β2-integrins were not effective. These data suggested that cell-to-cell communication mediated by E-cadherin contributes to the acquirement of a cardiomyogenic phenotype of human endothelial progenitor cells.
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U2 - 10.1016/j.febslet.2005.09.071
DO - 10.1016/j.febslet.2005.09.071
M3 - Article
C2 - 16226258
AN - SCOPUS:27544461543
SN - 0014-5793
VL - 579
SP - 6060
EP - 6066
JO - FEBS Letters
JF - FEBS Letters
IS - 27
ER -